Nucleic-acid binding by tetracycline·metal ion complexes

Bioinorganic chemistry Pub Date : 1978-01-01 DOI:10.1016/S0006-3061(00)80150-7

Peter Mikelens, Warren Levinson

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引用次数: 13

Abstract

Tetracycline forms chelate complexes with cupric, nickelous, and cobaltous ions that bind DNA when analyzed in a filter-retention assay. Tetracycline complexes with other metal ions including zinc, ferrous, ferric, manganous, magnesium, and calcium ions do not produce this effect. The tetracycline·copper complex binds the homopolymers polyriboadenylate, polyribouridylate, polyriboinosinate, and polyribocytidylate. The binding of polyribocytidylate is least effective. The preaddition of calcium ions interferes with the ability of tetracycline to form a DNA-binding complex with cupric ions. Calcium ions do not block DNA binding by an already formed tetracycline·copper complex.

Riboflavin interferes with the DNA-binding action of tetracycline in the filter-retention assay. This suggests a rationale for its reported antagonism of bacterial growth inhibition by tetracycline. Riboflavin does not block the DNA-binding activity of an already formed tetracycline·copper complex. None of the riboflavin·metal ion complexes tested are capable of binding DNA in the filter-retention assay.

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四环素·金属离子配合物的核酸结合

四环素与铜、镍和钴离子形成螯合络合物，在过滤保留试验中结合DNA。四环素与其他金属离子包括锌、铁、铁、锰、镁和钙离子的配合物不会产生这种效果。四环素·铜络合物结合均聚物聚核糖苷酸、聚核糖苷酸、聚核糖苷酸和聚核糖苷酸。多核苷酸结合效果最差。预先加入钙离子会干扰四环素与铜离子形成dna结合复合物的能力。钙离子不会阻断已经形成的四环素·铜复合物的DNA结合。核黄素在过滤保留试验中干扰四环素的dna结合作用。这提示了其报道的四环素对细菌生长抑制的拮抗作用的基本原理。核黄素不会阻断已经形成的四环素·铜复合物的dna结合活性。在过滤保留试验中，核黄素·金属离子配合物均不能与DNA结合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Bioinorganic chemistry

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