Green Nanotechnology from Brassicaceae: Development of Broccoli Phytochemicals–Encapsulated Gold Nanoparticles and Their Applications in Nanomedicine

International Journal of Green Nanotechnology Pub Date : 2013-01-01 DOI:10.1177/1943089213509474

M. Khoobchandani, Ajit P. Zambre, K. Katti, Chung-Ho Lin, K. Katti

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引用次数: 29

Abstract

The interaction of cocktail of phytochemicals from broccoli with gold salt results in dual reduction and surface capping to produce well-defined stable and biocompatible gold nanoparticles (B-AuNPs). Broccoli phytochemicals–coated gold nanoparticles (B-AuNPs) have been fully characterized. Detailed in vitro stability in various biological fluids and affinity and selectivity for tumor cells have been investigated. The B-AuNPs showed significant in vitro cytotoxic effects against various cancer cells (MDA-MB-231, PC-3, U266, SkBr3, and T47D) as confirmed by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) and flow cytometry apoptosis assays. Surface encapsulation of cocktail of broccoli phytochemicals on AuNPs facilitates the cellular internalization, thereby validating the in vitro therapeutic effects of these nanoparticles. Detailed analyses performed by combination of gas chromatography–mass spectrometry (GC–MS) and liquid chromatography–tandem mass spectrometry (LC–MS–MS) have confirmed the ...

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甘蓝科绿色纳米技术:花椰菜植物化学物质包封金纳米颗粒的开发及其在纳米医学中的应用

花椰菜中的植物化学物质与金盐的混合物相互作用导致双重还原和表面盖帽，从而产生定义明确、稳定且具有生物相容性的金纳米粒子(B-AuNPs)。花椰菜植物化学物质包被的金纳米颗粒(B-AuNPs)已经被完全表征。详细的体外稳定性在各种生物液体和亲和力和选择性肿瘤细胞进行了研究。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯四唑(MTT)和流式细胞术细胞凋亡实验证实，B-AuNPs对多种癌细胞(MDA-MB-231、PC-3、U266、SkBr3和T47D)具有显著的体外细胞毒作用。西兰花植物化学物质混合物表面包封在AuNPs上，促进了细胞内化，从而验证了这些纳米颗粒的体外治疗效果。通过气相色谱-质谱联用(GC-MS)和液相色谱-串联质谱联用(LC-MS-MS)进行了详细的分析，证实了

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International Journal of Green Nanotechnology

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