Xia Cai, Jiaxin Qin, Xuelian Li, Taoxiong Yuan, Bing Yan, Jun Cai
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引用次数: 0
Abstract
Intracellular pH critically affects various biological processes, and an appropriate cytoplasmic pH is essential for ensuring bacterial growth. Glucose is the preferred carbon source for most heterotrophs; however, excess glucose often causes the accumulation of acidic metabolites, lowering the intracellular pH and inhibiting bacterial growth. Bacillus thuringiensis can effectively cope with glucose-induced stress; unfortunately, little is known about the regulators involved in this process. Here, we document that the target of the dual-function sRNA YhfH, the lipR gene, encodes a LacI-family transcription factor LipR as an intracellular pH regulator when B. thuringiensis BMB171 is suddenly exposed to glucose. Under glucose conditions, lipR deletion leads to early growth arrest by causing a rapid decrease in intracellular pH (~5.4). Then, the direct targets and a binding motif (GAWAWCRWTWTCAT) of LipR were identified based on the electrophoretic mobility shift assay, the DNase-I footprinting assay, and RNA sequencing, and the gapN gene encoding a key enzyme in glycolysis was directly inhibited by LipR. Furthermore, Ni2+ is considered a possible effector for LipR. In addition to YhfH, the lipR expression was coregulated by itself, CcpA, and AbrB. Our study reveals that LipR plays a balancing role between glucose metabolism and intracellular pH in B. thuringiensis subjected to glucose stress.
期刊介绍:
Since 1929 The Journal of the Acoustical Society of America has been the leading source of theoretical and experimental research results in the broad interdisciplinary study of sound. Subject coverage includes: linear and nonlinear acoustics; aeroacoustics, underwater sound and acoustical oceanography; ultrasonics and quantum acoustics; architectural and structural acoustics and vibration; speech, music and noise; psychology and physiology of hearing; engineering acoustics, transduction; bioacoustics, animal bioacoustics.