LipR functions as an intracellular pH regulator in Bacillus thuringiensis under glucose conditions.

IF 2.1 2区 物理与天体物理 Q2 ACOUSTICS
Journal of the Acoustical Society of America Pub Date : 2023-02-11 eCollection Date: 2023-03-01 DOI:10.1002/mlf2.12055
Xia Cai, Jiaxin Qin, Xuelian Li, Taoxiong Yuan, Bing Yan, Jun Cai
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Abstract

Intracellular pH critically affects various biological processes, and an appropriate cytoplasmic pH is essential for ensuring bacterial growth. Glucose is the preferred carbon source for most heterotrophs; however, excess glucose often causes the accumulation of acidic metabolites, lowering the intracellular pH and inhibiting bacterial growth. Bacillus thuringiensis can effectively cope with glucose-induced stress; unfortunately, little is known about the regulators involved in this process. Here, we document that the target of the dual-function sRNA YhfH, the lipR gene, encodes a LacI-family transcription factor LipR as an intracellular pH regulator when B. thuringiensis BMB171 is suddenly exposed to glucose. Under glucose conditions, lipR deletion leads to early growth arrest by causing a rapid decrease in intracellular pH (~5.4). Then, the direct targets and a binding motif (GAWAWCRWTWTCAT) of LipR were identified based on the electrophoretic mobility shift assay, the DNase-I footprinting assay, and RNA sequencing, and the gapN gene encoding a key enzyme in glycolysis was directly inhibited by LipR. Furthermore, Ni2+ is considered a possible effector for LipR. In addition to YhfH, the lipR expression was coregulated by itself, CcpA, and AbrB. Our study reveals that LipR plays a balancing role between glucose metabolism and intracellular pH in B. thuringiensis subjected to glucose stress.

在葡萄糖条件下,LipR 在苏云金芽孢杆菌中发挥细胞内 pH 值调节器的功能。
细胞内 pH 值对各种生物过程有着至关重要的影响,适当的细胞质 pH 值对确保细菌生长至关重要。葡萄糖是大多数异养生物的首选碳源;然而,过量的葡萄糖往往会导致酸性代谢产物的积累,从而降低细胞内 pH 值,抑制细菌生长。苏云金芽孢杆菌能有效地应对葡萄糖诱导的应激;遗憾的是,人们对这一过程中涉及的调节因子知之甚少。在这里,我们发现当苏云金芽孢杆菌 BMB171 突然暴露于葡萄糖时,双重功能 sRNA YhfH 的靶基因 lipR 编码 LacI 家族转录因子 LipR,作为细胞内 pH 值的调节因子。在葡萄糖条件下,缺失 LipR 会导致细胞内 pH 值迅速降低(~5.4),从而导致早期生长停滞。随后,根据电泳迁移试验、DNase-I footprinting 试验和 RNA 测序,确定了 LipR 的直接靶标和结合基序(GAWAWCRWTWTCAT),并发现 LipR 直接抑制了编码糖酵解关键酶的 gapN 基因。此外,Ni2+ 被认为是 LipR 的一个可能效应因子。除 YhfH 外,LipR 的表达还受到其自身、CcpA 和 AbrB 的核心调节。我们的研究揭示了 LipR 在葡萄糖胁迫下的苏云金芽孢杆菌的葡萄糖代谢和细胞内 pH 之间起着平衡作用。
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来源期刊
CiteScore
4.60
自引率
16.70%
发文量
1433
审稿时长
4.7 months
期刊介绍: Since 1929 The Journal of the Acoustical Society of America has been the leading source of theoretical and experimental research results in the broad interdisciplinary study of sound. Subject coverage includes: linear and nonlinear acoustics; aeroacoustics, underwater sound and acoustical oceanography; ultrasonics and quantum acoustics; architectural and structural acoustics and vibration; speech, music and noise; psychology and physiology of hearing; engineering acoustics, transduction; bioacoustics, animal bioacoustics.
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