Efficacy of therapeutic nanoformulations in a collagen-based three-dimensional colorectal cancer in vitro model

Abstract

Methods: AZD6244-containing GCPQ micelles and DOPE/DC-cholesterol liposomes were fabricated using sonication and extrusion methods respectively. These formulations were tested together with the free drug in HCT116 cell monolayers and a collagen-based 3D cancer model to determine differences in efficacy between both models. Fluorescent nanoparticles were used to determine their ability to penetrate the scaffold and reach its core.