Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

Medical Photonics Pub Date : 2015-05-01 DOI:10.1016/j.medpho.2014.12.001

Klaus Suhling , Liisa M. Hirvonen , James A. Levitt , Pei-Hua Chung , Carolyn Tregidgo , Alix Le Marois , Dmitri A. Rusakov , Kaiyu Zheng , Simon Ameer-Beg , Simon Poland , Simao Coelho , Robert Henderson , Nikola Krstajic

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引用次数: 198

Abstract

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is independent of the local fluorophore concentration and excitation intensity. One prominent FLIM application relevant for biological concerns is the identification of FRET to study protein interactions and conformational changes, but FLIM is also used to image viscosity, temperature, pH, refractive index and ion and oxygen concentrations, all at the cellular level, as well as cell and tissue autofluorescence. The basic principles and recent advances in the application of FLIM, FLIM instrumentation, data analysis, molecular probe and FLIM detector development will be discussed.

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荧光寿命成像(FLIM):基本概念和最新进展

荧光寿命成像(FLIM)是一种用于绘制环境和荧光探针相互作用的关键荧光显微技术。它可以报道荧光强度成像难以或不可能观察到的光物理事件，因为FLIM不依赖于局部荧光团浓度和激发强度。与生物学相关的一个突出的FLIM应用是鉴定FRET来研究蛋白质相互作用和构象变化，但FLIM也用于成像粘度、温度、pH、折射率、离子和氧浓度，所有这些都在细胞水平上，以及细胞和组织的自身荧光。本文将讨论FLIM的基本原理和最新应用进展、FLIM仪器、数据分析、分子探针和FLIM探测器的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Medical Photonics

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