SIMULTANEOUS DETERMINATION OF KREBS CYCLE ACIDS BY GAS-LIQUID CHROMATOGRAPHY.

A. Kuksis, P. Vishwakarma
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引用次数: 19

Abstract

Carbowax-coated Teflon powder (2–3%, w/w, on Teflon 6, 90–100 mesh) columns (5 ft long, 1/8 in. diam.) were used with the Aerograph Hy-Fi gas chromatograph. Complete separations and symmetric peaks were obtained both isothermally (150 °C) and by temperature programming (100–160 °C) for the methyl esters of succinic, oxalacetic, malic, alpha-ketoglutaric, trans- and cis-aconitic, and citric acids. On account of enolization, the alpha-keto esters gave more than one peak, the proportions of which varied with the chromatographic conditions. The succinic and fumaric esters overlapped. The trans isomers were eluted ahead of the cis isomers.A linear relationship was obtained between the areas recorded and the quantities of the individual esters injected in the range of 0.01–10 μg. The absolute response varied with the oxygen content of each ester. Analysis of polycarboxylic acid mixtures from natural sources gave values comparable to those obtained by the more laborious conventional techniques.
气液色谱法同时测定克雷布斯循环酸。
碳蜡涂层聚四氟乙烯粉末(2-3%,w/w,聚四氟乙烯6,90 - 100目)柱(5英尺长,1/8英寸。)与Aerograph Hy-Fi气相色谱仪一起使用。琥珀酸甲酯、草酸乙酸甲酯、苹果酸甲酯、α -酮戊二酸甲酯、反式和顺式乌头酸甲酯和柠檬酸甲酯在等温(150℃)和温度编程(100-160℃)下均获得完全分离和对称峰。由于烯醇化作用,α -酮酯出现了多个峰,其比例随色谱条件的不同而变化。琥珀酸酯和富马酸酯重叠。反式异构体先于顺式异构体洗脱。在0.01 ~ 10 μg范围内,所记录的面积与单个酯的注射量呈线性关系。绝对响应随各酯的氧含量而变化。对天然来源的聚羧酸混合物的分析得出的值与更费力的传统技术所得的值相当。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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