The Involvement of lncRNA HOTAIR/miR-130a-3p Axis in the Regulation of Voluntary Exercise on Cognition and Inflammation of Alzheimer's Disease.

American Journal of Alzheimer's Disease & Other Dementias® Pub Date : 2022-01-01 DOI:10.1177/15333175221091424

Jianxia Lu, Lihua Liu, Jin Chen, Juan Zhi, Jiabin Li, Le Li, Zhongli Jiang

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Abstract

Background: Alzheimer's disease (AD) is an age-related neurodegenerative disease and exercises might mitigate the progression of AD. This investigation aimed to manifest the potential mechanism of exercises in AD.

Methods: Morris water maze (MWM) test was conducted to evaluate the cognitive function in APP/PS1 mice. Quantitative real-time PCR was performed to detect the expression of HOTAIR and miR-130a-3p. The enzyme-linked immunosorbent assay was applied to appraise the concentration of IL-1β, IL-6, and TNF-α. A luciferase report experiment was implemented to substantiate the relationship between miR-130a-3p and HOTAIR.

Results: Exercises contributed to the elevated expression of HOTAIR. The findings of MWM implied HOTAIR inhibited the impacts of voluntary exercises on escape latency, distance moved, percentage of time spent in the target quadrant, platform crossing times, and inflammation. MiR-130a-3p mediated the function of HOTAIR on cognitive ability and inflammation.

Conclusion: HOTAIR participated in the regulation of exercises on AD by sponging miR-130a-3p.

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lncRNA HOTAIR/miR-130a-3p 轴参与自主运动对阿尔茨海默病认知和炎症的调控

背景：阿尔茨海默病（AD）是一种与年龄相关的神经退行性疾病，运动可能会缓解AD的进展。本研究旨在揭示运动治疗阿尔茨海默病的潜在机制：方法：采用莫里斯水迷宫（MWM）测试评估APP/PS1小鼠的认知功能。定量实时 PCR 检测 HOTAIR 和 miR-130a-3p 的表达。酶联免疫吸附试验检测了IL-1β、IL-6和TNF-α的浓度。通过荧光素酶报告实验证实了 miR-130a-3p 与 HOTAIR 之间的关系：结果：运动促进了 HOTAIR 的表达。MWM的研究结果表明，HOTAIR抑制了自主运动对逃逸潜伏期、移动距离、目标象限停留时间百分比、平台穿越时间和炎症的影响。MiR-130a-3p介导了HOTAIR对认知能力和炎症的作用：结论：HOTAIR通过疏导miR-130a-3p参与了运动对AD的调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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American Journal of Alzheimer's Disease & Other Dementias®

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