Quality control analysis of Safoof-e-Pathar phori: Antiurolithiatic formulation

Drug Development and Therapeutics Pub Date : 2016-01-01 DOI:10.4103/2394-6555.180163

W. Ahmad, W. Khan, M. Khan, Mohd Mujeeb, S. Arif Zaidi, Sayeed Ahmad

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引用次数: 4

Abstract

Aim: Safoof-e-Pathar phori (SPP), a Unani polyherbomineral formulation used for antilithiatic activity. The present study involves standardization of SPP to assess the quality. SPP were subjected to pharmacognostic studies, physiochemical properties, phytochemical analysis, high-performance thin layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC-MS) fingerprint profile to set the standards, which can be used as reference of quality by herbal industries for its preparation and human use. Materials and Methods: The quality control of SPP has been carried out as per the AYUSH and WHO guidelines. The HPTLC fingerprinting has been done using chloroform: ethyl acetate (9:2, v/v) for petroleum ether extract, chloroform: toluene: ethyl acetate (7:2:4, v/v/v) for chloroform extract and for methanol extract petroleum ether: ethyl acetate (9:2, v/v) was used as mobile phase. HPLC was carried out using mobile phase consisted of acetonitrile and water in the ratio of 50:50 (v/v) for the methanolic extract. GC-MS fingerprinting profile has been carried out using hexane extract. Result: SPP was subjected to qualitative estimation of phytochemicals using standard methods, which revealed the presence of various bioactive components such as anthraquinone glycosides, carbohydrates, resins, proteins, flavonoids, phenolics, tannins, and terpenoids. The quantitative estimation of total phenolics and flavonoid content showed 0.44 mg/g and 1.02 mg/g, respectively. The HPTLC fingerprint showed presence of number of compounds for extracts at different Rfvalues. However, HPLC fingerprinting showed presence of 23 well-separated compounds and GC-MS showed presence of 22 compounds. Conclusion: The quality control parameters in present study reveal complete standardization profile of SPP for the 1st time, which would be of immense value in checking quality of developed formulation for human use.

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抗尿石剂Safoof-e-Pathar - phori的质量控制分析

目的:研究一种具有抗石活性的乌纳尼多矿物制剂——萨弗夫-帕塔佛里(SPP)。本研究涉及SPP的标准化，以评估质量。通过生药学研究、理化性质、植物化学分析、高效薄层色谱(HPTLC)、高效液相色谱(HPLC)、气相色谱-质谱(GC-MS)指纹图谱等方法建立标准，为中药行业制备和人用中药提供质量参考。材料和方法:SPP的质量控制按照AYUSH和WHO指南进行。以氯仿:乙酸乙酯(9:2,v/v)为流动相，以氯仿:甲苯:乙酸乙酯(7:2:4,v/v/v)为流动相，以石油醚:乙酸乙酯(9:2,v/v)为流动相进行hplc指纹图谱分析。甲醇提取物以乙腈和水为流动相，以50:50 (v/v)的比例进行高效液相色谱法。采用己烷提取物进行了气相色谱-质谱指纹图谱分析。结果:采用标准方法对SPP进行了植物化学成分的定性鉴定，发现SPP中含有蒽醌苷类、碳水化合物、树脂、蛋白质、黄酮类、酚类、单宁类、萜类等多种生物活性成分。总酚和类黄酮含量分别为0.44 mg/g和1.02 mg/g。HPTLC指纹图谱显示，在不同的rf值下，提取物中存在较多的化合物。HPLC指纹图谱鉴定出23个分离良好的化合物，GC-MS鉴定出22个化合物。结论:本研究的质量控制参数首次揭示了SPP的完整标准化概况，对开发的人用制剂的质量检验具有重要价值。

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Drug Development and Therapeutics

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