Genetic Comparison Between Coat Protein Gene of Alfalfa mosaic virus Isolate Infecting Potato Crop in Upper Egypt and Worldwide Isolates

O. Abdalla, Safynaz A. Mohamed, A. Eraky, F. G. Fahmay
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引用次数: 9

Abstract

Alfalfa mosaic virus is one of the most important viruses infecting potato worldwide. Genetic comparison between Coat Protein (CP) gene of AMV isolate infecting potato in Upper Egypt (AMV-Assiut) and worldwide isolates was carried out in this study. The AMV-Assiut isolate shared similarity in CP gene ranged from 90-95 and 95-97% in nucleotide and amino acid sequences of CP gene, respectively. The AMV-Assiut shared the highest similarity with Egyptian AMV isolates (Wady Elnatron) and AMV isolate from Croatia in case of nucleotide and amino acid, respectively. Phylogenetic analysis showed that AMV isolates tend to cluster into two main groups, with additional clustering of AMV isolates in each group into two subgroups, supporting the hypothesis of existence two main strains of AMV. No clear geographical or host origin structure was found among AMV isolates.
上埃及马铃薯苜蓿花叶病毒分离物与世界各地分离物外壳蛋白基因的遗传比较
苜蓿花叶病毒是马铃薯最重要的侵染病毒之一。本研究对上埃及马铃薯AMV分离株(AMV- assiut)的外壳蛋白(CP)基因与世界各地的AMV分离株进行了遗传比较。AMV-Assiut分离物在CP基因核苷酸和氨基酸序列上的相似性分别为90- 95%和95-97%。AMV- assiut分别与埃及AMV分离株(Wady Elnatron)和克罗地亚AMV分离株在核苷酸和氨基酸方面具有最高的相似性。系统发育分析表明,AMV分离株倾向于聚集为两个主要类群,每个类群中的AMV分离株又聚集为两个亚类群,支持AMV存在两个主菌株的假设。在AMV分离株中没有发现明确的地理或宿主来源结构。
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