Isotonic Solutions XI*

Abstract

The effects of various sympathomimetic amine salts and phenothiazine derivatives in preventing hemolysis of human erythrocytes were studied. In some instances rabbit erythrocytes were used. The results indicated that in solutions of increasing concentrations of sympathomimetic amine salts, hemolysis initially decreased, then abruptly increased, followed by a second greater decrease and increase. In the presence of 0.6 per cent sodium chloride there was no hemolysis until a certain concentration of sympathomimetic amine salt was reached, which closely coincided with the concentration of the same drug that resulted in the final increase in hemolysis when sodium chloride was not present. Freezing point depressions and pH values of the compounds employed in the hemolytic method were determined. The compounds were tested for reactions with human serum and oxyhemoglobin. The phenothiazine derivatives tested were found to be unsuited for investigation by the hemolytic method.