ANTIMICROBIAL ACTIVITY OF ETHANOL EXTRACTS OF $RIBES~NIGRUM$ AND $RIBES~RUBRUM$ LEAVES

Proceedings of the YSU B: Chemical and Biological Sciences Pub Date : 2023-03-27 DOI:10.46991/pysu:b/2023.57.1.054

Anush M. Babayan, N. Sahakyan

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Abstract

Antimicrobial activity of Ribes nigrum L. and Ribes rubrum L. leaf extracts was determined by disk-diffusion method as well as specific growth rate and generation succeeding factor determination. Different Gram-positive (Bacillus subtilis WT-A, Staphylococcus aureus MDC 5233, Enterococcus hirae ATCC 9790) and Gram-negative (Escherichia coli ATCC 25922, Salmonella typhimurium MDC1754, ampicillin resistant E. coli dhpα-pUC18 and kanamycin resistant E. coli pARG25) bacteria and yeasts (Saccharomyces cerevisiae ATCC 9804 and S. cerevisiae ATCC 13007) were used as test-microorganisms. 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was applied to measure the radical scavenging capacity of extracts obtained from Ribes spp. R. rubrum and R. nigrum extracts antiradical activity expressed with IC50 value of 91.2±1.69 µg‧mL–1 and 66.01±1.65 µg‧mL–1, respectively. The total flavonoid content in plant extracts was determined employing AlCl3 colorimetric assay, and the values were 34.71±0.63 and 49.99±0.86 μg QE‧mg–1 for R. rubrum and R. nigrum extracts, respectively. Total phenolic content of studied extracts was investigated by Folin–Ciocalteu assay. The contents for R. rubrum and R. nigrum extracts were 133.12±6.65 and 167.15±7.29 μg GAE‧mg–1, respectively. Thus, these plants can be considered as potential sources of biologically active substances.

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荆芥叶和荆芥叶乙醇提取物的抑菌活性研究

采用圆盘扩散法、比生长率法和代继承因子法测定了黑荆芥和红荆芥叶提取物的抑菌活性。以革兰氏阳性菌(枯草芽孢杆菌WT-A、金黄色葡萄球菌MDC 5233、霍乱肠球菌ATCC 9790)和革兰氏阴性菌(大肠杆菌ATCC 25922、鼠伤寒沙门氏菌MDC1754、耐氨青霉素大肠杆菌dhpα-pUC18和耐卡那霉素大肠杆菌pARG25)和酵母(酿酒酵母ATCC 9804和酿酒酵母ATCC 13007)作为试验微生物。采用1,1-二苯基-2-苦味酰肼(DPPH)法测定了红小檗和黑小檗提取物的自由基清除能力，IC50值分别为91.2±1.69µg·mL-1和66.01±1.65µg·mL-1。采用AlCl3比色法测定植物提取物中总黄酮的含量，结果表明，红小檗和黑小檗提取物的总黄酮含量分别为34.71±0.63和49.99±0.86 μg QE·mg-1。采用Folin-Ciocalteu法测定提取物中总酚的含量。红小檗和黑小檗提取物的含量分别为133.12±6.65和167.15±7.29 μg GAE·mg-1。因此，这些植物可以被认为是生物活性物质的潜在来源。

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Proceedings of the YSU B: Chemical and Biological Sciences

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