Antimicrobial Activity of Angelica Dahurica Ethanol Extract Against Oral Bacteria

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Abstract

Introduction: Angelica dahurica (A. dahurica) has the protective activity against dexamehasone-induced disorders, liver protective activity, antimutagenic activity, anti-inflammatory, anti-microbial, anti-oxidative, anti-asthmatic, and anti-cancer effects. Aims of the Study: This study aimed to investigate the synergistic antibacterial activity with existing antimicrobial agents against oral pathogen. Materials and Methods: The synergistic effects of 50% ethanol extract of A. dahurica (ADEE) were evaluated against oral bacteria, either alone or with antibiotics, via broth microdilution and time-kill method. Results: The minimal inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) values for ADEE, ampicillin and gentamicin against all the tested bacteria ranged between 62.5-1000/250-2000 μg/mL, 0.0625-16/0.25-32 μg/mL, and 4-128/16-256 μg/mL, respectively. The ADEE displayed synergism with ampicillin and gentamicin, with 8-fold reductions in the MIC/MBC. Furthermore, a time-kill study showed that the growth of the tested bacteria was completely attenuated after treatment with 1/2 MIC of ADEE with 1/2 MIC of antibiotics resulted from an increase of the rate of killing in units of CFU/mL to a greater degree than was observed with alone. Discussion and Conclusions: The results of this study demonstrate the antimicrobial and synergistic activity of ADEE and antibiotics against oral pathogens.
白芷乙醇提取物对口腔细菌的抑菌活性研究
白芷(A. dahurica)具有抗地塞米松性疾病、保护肝脏、抗诱变、抗炎、抗微生物、抗氧化、平喘、抗癌等作用。研究目的:研究其与现有抗菌药物对口腔病原菌的协同抑菌活性。材料与方法:采用微量肉汤稀释法和时间杀伤法,考察了50%乙醇提取物(ADEE)单独或与抗生素联合对口腔细菌的增效作用。结果:ADEE、氨苄西林和庆大霉素对所有细菌的最小抑菌浓度(mic)和最小杀菌浓度(MBCs)分别为62.5 ~ 1000/250 ~ 2000 μg/mL、0.0625 ~ 16/0.25 ~ 32 μg/mL和4 ~ 128/16 ~ 256 μg/mL。ADEE显示出与氨苄西林和庆大霉素的协同作用,MIC/MBC降低了8倍。此外,一项时间杀伤研究表明,1/2 MIC的ADEE和1/2 MIC的抗生素处理后,被试细菌的生长完全减弱,单位为CFU/mL的杀灭率比单独使用的杀灭率增加得更大。讨论与结论:本研究结果证明了ADEE与抗生素对口腔病原菌的抑菌和协同作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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