Sensitivity assessment of Phytophthora nicotianae to fungicides of fluazinam and its mixture with carboxylic acid amide

Mu Wenjun, L. Qi, Chen Xiuhua, Liao Haijiang, Dong Kun-le, Wang Jianwei, Zhang Youwu, Feng Xiaohu, Zhang Zhigao, W. Libing, Song Jizhen
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Abstract

Tobacco black shank caused by Phytophthora parasitica var. nicotianae (Breda de Hean) is one of the most serious diseases in tobacco growing, and few effective fungicides are available currently. The purpose of this study was to assess the feasibility of using fluazinam to treat tobacco black shank disease. In vitro toxicities of fluazinam to 62 isolates of P. nicotianae were determined by mycelial growth rate method. The EC50 values ranged from 0.294 6 to 2.389 9 μg/mL with a mean of 1.084 0 μg/mL. The sensitivity distribution was unimodal, and there was no resistant isolate in P. nicotianae population. The carboxylic acid amide (CAA) fungicides were single-site in action and activity to oomycetes. The synergistic interactions were tested by Wadley method between fluazinam and dimethomorph, flumorph, mandipropamid, respectively. The mixture containing dimethomoph:fluazinam at 1:2 ratio showed additive actions, with the synergy ratio of 1.2. The mixture containing flumorph and fluazinam at 1:5 ratio had the highest synergy ratio (SR 2.07). Mandipropamid and fluazinam had an additive interaction and the proportion of 2:1 had the lowest EC50 value (0.020 7 μg/mL). This study demonstrated the sensitivity of P. nicotianae to fluazinam, and provided the experimental foundation to use fluazinam for tobacco black shank management.
烟草疫霉对氟西南及其与羧酸酰胺复配杀菌剂的敏感性评价
烟草黑胫病(Phytophthora parasitica var. nicotianae, Breda de Hean)是烟草种植业最严重的病害之一,目前有效的杀菌剂很少。本研究的目的是评价氟西南治疗烟草黑胫病的可行性。采用菌丝生长速率法测定了氟西南对62株烟草假单胞菌的体外毒力。EC50值范围为0.294 6 ~ 2.389 9 μg/mL,平均值为1.084 0 μg/mL。该菌株的敏感性呈单峰型分布,种群中未发现耐药菌株。羧酸酰胺(CAA)杀菌剂对卵菌具有单位点作用和活性。采用Wadley法检测氟西南与吡虫啉、氟虫啉、下颌丙胺的协同作用。以1:2的比例与氟唑西南混合后,具有加性作用,协同比为1.2。氟虫啉与氟西南以1:5的比例混合,协同率最高(SR为2.07)。丙咪酰胺与氟西南呈加性相互作用,比例为2:1时EC50值最低(0.020 7 μg/mL)。本研究证实了烟草假单胞菌对氟唑西南的敏感性,为氟唑西南在烟草黑胫病防治中的应用提供了实验依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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