Headspace volatiles of Chaerophyllum aureum L.

Abstract

Differences in the headspace volatile profiles (HS) of fresh and air-dried fruits, stems and aerial parts of Chaerophyllum aureum L. (Apiaceae) were studied here for the first time using HS-GC-MS (head space – gas chromatography – mass spectrometry). This was done in order to probe to which level HS volatiles of different plant organs were susceptible to air drying. The most dominant headspace volatiles of all samples were monoterpene hydrocarbons. Sabinene was the major volatile of the fresh aerial parts, air-dried fruits, fresh and air-dried stems, representing 47.8%, 31.4%, 67.7% and 73.0% of the total volatiles, respectively. The most abundant headspace volatiles of the fresh fruits were terpinolene (45.3%), γ-terpinene (13.1%) and β-pinene (10.2%). The air-dried aerial parts were characterized by a high amount of limonene (69.0%). The results of HS-GC-MS were subjected to multivariate statistical analysis in order to get a better insight into the similarities/dissimilarities existing between the investigated samples. According to the results of multivariate analysis, the drying process significantly influenced HS volatiles. ISPARLJIVI SASTOJCI BILJNE VRSTE CHAEROPHYLLUM AUREUM L. U ovom radu su, po prvi put, ispitane razlike u headspace profilima (HS) svežih i na vazduhu susenih plodova, stabljika i nadzemnih delova biljne vrste Chaerophyllum aureum L (Apiaceae) pomocu metode HS-GC-MS (headspace – gasna hromatografija – masena spektrometrija). Na osnovu ovih rezultata moglo bi se utvrditi do koliko intenzivnih promena u HS profilima razlicitih delova biljke može doci usled susenja biljnog materijala. U svim uzorcima su dominantni headspace isparljivi sastojci bili monoterpeni. Utvrđeno je da je sabinen bio glavni isparljivi sastojak svežeg nadzemnog dela (47,8%), suvog ploda (31,4%), svežeg (67,7%) i suvog stabla (73,0%). Kao glavni isparljivi sastojci svežeg ploda nađeni su terpinolen (45,3%), γ-terpinen (13,1%) i β-pinen (10,2%), dok je suvi nadzemni deo bio okarakterisan znacajno vecom zastupljenoscu limonena (69,0%). Rezultati HS-GC-MS analize su podvrgnuti multivarijantnoj statistickoj analizi kako bi se dobio bolji uvid u slicnosti/razlike među razmatranim uzorcima. Na osnovu rezultata multivarijantne analize sledi da susenje u velikoj meri utice na HS profil. HIGHLIGHTS Headspace (HS) profiles (percentage compositions) of different parts of Chaerophyllum aureum L. were subjected to multivariate statistical analysis. In all samples, fresh and dry, the most dominant headspace volatiles were monoterpene hydrocarbons. Sabinene was the most abundant volatile of the fresh aerial parts, air-dried fruits, fresh and air-dried stems. The main headspace volatile compound of the fresh fruits was terpinolene while limonene was the major volatile of the air-dried aerial parts.