Preparation And Evaluation of Hydrogel Formulation Containing Ocimum Sanctum Leaves Extract for Anti-Inflammatory Activity

A. Jain
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Abstract

Aim: Aim of the present study was to evaluate anti-inflammatory effect of Ocimum sanctum leaves in the formulation of hydrogel using different types of animal models. Materials and Methods: Ethanolic extract of O. sanctum leaves was fractionated with methanol to get methanolic fraction. Methanolic fraction (1% vw/w) was used for hydrogel formulation with different combination of Carbopol 940 and Sodium CMC. Prepared hydrogel was characterized for optimum physical characters, pH, spreadability, homogeneity, viscosity, release profile, and irritation effect. Optimized hydrogel was evaluated for anti-inflammatory effect using xylene-induced, croton oilinduced ear edema, and cotton pellet-induced granuloma model in animals. Effect was monitored by measurement of percent inhibition, nitric oxide (NO), and myeloperoxidase (MPO) levels in mice ears after acute inflammation induced by croton oil. Results and Discussion: Physical evaluation confirmed the color of the prepared hydrogels was brownish and appearance was homogeneous and smooth on application. All other evaluation parameters, for example, pH, viscosity, spreadability, and consistency were found suitable in F3 hydrogel formulation combination. Hence, the optimize composition of F3 formulation was observed as 1.5 g of Carbopol 940 and 1% of Sodium CMC. Percent inhibition of edema in xylene induced ear edema in mice was found comparable to standard group of treatment (65.59%). In the present study, result indicates that prepared hydrogel hydrogel of methanol fraction of O. sanctum possesses inhibitory effects against acute inflammation. The results showing the ability of croton oil to induce neutrophil influx into mice ear tissue. This may be reduced by treatment with extract hydrogels, as well as Voltaren Emulgel, reduced significantly (P<0.01) the MPO levels and NO levels in mice ears. Conclusion: The methanol extracts was exhibits its anti-inflammatory action significantly may be due to the presence of flavonoids in O. sanctum leaves.
茴香叶提取物水凝胶制剂的制备及抗炎活性评价
目的:采用不同类型的动物模型,对复方水凝胶中桑叶的抗炎作用进行评价。材料与方法:用甲醇对荆芥叶乙醇提取物进行分馏,得到甲醇馏分。以甲醇馏分(1% vw/w)为水凝胶剂,以卡波波940与CMC钠的不同组合配制水凝胶。对制备的水凝胶进行了物理特性、pH值、涂抹性、均匀性、粘度、释放特性和刺激效果的表征。采用二甲苯致耳部水肿、巴豆油致耳部水肿和棉球致肉芽肿模型对优化后的水凝胶进行抗炎作用评价。通过测量巴豆油引起急性炎症后小鼠耳内抑制百分比、一氧化氮(NO)和髓过氧化物酶(MPO)水平来监测效果。结果与讨论:物理评价证实制备的水凝胶颜色为褐色,使用时外观均匀光滑。所有其他评价参数,如pH、粘度、铺展性和稠度,均适用于F3水凝胶配方组合。因此,F3配方的最佳配比为卡波波尔940 1.5 g、CMC钠1%。二甲苯诱导小鼠耳部水肿的抑制率与标准组相当(65.59%)。在本研究中,结果表明,制备的水凝胶,水凝胶的甲醇部分,对急性炎症有抑制作用。结果显示,巴豆油诱导中性粒细胞流入小鼠耳组织的能力。水凝胶和伏他仑乳凝胶可显著降低小鼠耳内MPO和NO水平(P<0.01)。结论:甲醇提取物具有明显的抗炎作用,可能与黄酮的存在有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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