Laser Auto Fluorescence and Dye-enhanced Spectroscopic Detection of Normal and Cancerous Human Lung Cell Lines and Plasmonic Photothermal Heating

Abstract

This work describes the results of laser autofluorescence, dye-enhanced fluorescence spectroscopy of normal MRC-5 and cancerous QU-DB human lung cell lines and the effect of gold nanoshells heating on the cells. It was shown that the intensity ratio of MRC-5 to QU-DB 80 (ΔR) and the shape factor (RP) can be appropriate indicators of normal and cancerous cells for primary and secondary peaks at 500 nm and 590 nm respectively. About 5 nm red shift was observed in the case of cancerous cells. Highest temperatures of about 51 and 39.5?C were achieved for (S3, S4) and (S5, S6) respectively. The percentage of damaged cells and nanoshells density are expected to decrease with radial distance from the center of the beam. The results also indicated an increasing degree of apoptosis with increasing the laser power, which critically governs the quality and the degree of laser-hyperthermia. The matrix temperature is thought to vary due to possible thermal overlapping of a larger fraction of NSs, agglomeration and clustering.