Production of polysaccharide-degrading enzymes by Septoria Nodorum in culture and during pathogenesis

Abstract

Septoria nodorum produced cell wall-degrading enzymes (CWDE) (oolygalacturonase (PG), xylanase (XY) and cellulase (CX)) both in mineral medium supplemented with wheat cell walls and in inoculated wheat leaves. The same in vitro and in vivo isoenzyme pattern for PG (pI 6.2 and 9.4) and XY (pI 5.2) was exhibited by isoelectric focusing resolution. CX was present in culture filtrates with one poorly resolved peak (pI 4.3–4.7). In infected tissues, an additional peak at pI 7.8 appeared. Some characteristics of S. nodorum polysaccharidases were compared with those of other leaf spot pathogens.