Escherichia Coli cell yield in media containing different carbohydrates

R. Alvin, Ambita Dave, Batalon Jamie Louise, Aba Berna Lou, Cortes Angelbert, Macabecha Cherray Gabrielle, M. Andrew
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Abstract

The objective of this study was to determine and compare the cell yield of Escherichia coli grown in minimal medium containing different concentrations of glucose (0.025%, 0.050% and 0.075%) and lactose (0.025%, 0.050% and 0.075%). Determination of the cell yield was done by oven drying the harvested cells to obtain the dry weight and analysis of the residual sugar in the liquid medium by phenol-sulfuric acid method. Highest initial concentration of glucose (596.667 ppm) and lactose (563.333 ppm) was recorded in minimal medium supplemented with 0.075% of the respective sugar. E. coli grown in 0.050% glucose (354.167 ppm) and 0.025% lactose (266.667 ppm) had the highest substrate consumed. Highest cell dry weight (μg/mL) and cell yield (μg/mL) was obtained in 0.075% glucose and 0.075% lactose. Comparing the two sugars, still, glucose had the highest cell yield (glucose 0.050% = 217.368 μg/mL and lactose 0.050% = 195.146 μg/mL; glucose 0.075% = 572.923 μg/ml and lactose 0.075% = 295.146 μg/ml). Highest Y was also recorded in highest sugar concentration (glucose 0.075% = 2.331; lactose 0.075% = 1.638). No statistical significance was observed when Y of different sugar concentrations were compared.
大肠杆菌细胞在含不同碳水化合物培养基中的产量
本研究的目的是确定并比较在含有不同浓度葡萄糖(0.025%、0.050%和0.075%)和乳糖(0.025%、0.050%和0.075%)的最小培养基中培养的大肠杆菌的细胞产量。细胞产量的测定是将收获的细胞用烘箱干燥得到干重,用苯酚-硫酸法分析液体培养基中的残糖。葡萄糖(596.667 ppm)和乳糖(563.333 ppm)的最高初始浓度分别记录在添加0.075%糖的最小培养基中。在0.050%葡萄糖(354.167 ppm)和0.025%乳糖(266.667 ppm)条件下生长的大肠杆菌消耗的底物最多。0.075%葡萄糖和0.075%乳糖条件下细胞干重(μg/mL)和细胞产量(μg/mL)最高。两种糖相比,葡萄糖的细胞产量最高(葡萄糖0.050% = 217.368 μg/mL,乳糖0.050% = 195.146 μg/mL);葡萄糖0.075% = 572.923 μg/ml,乳糖0.075% = 295.146 μg/ml)。糖浓度最高时Y值也最高(葡萄糖0.075% = 2.331;乳糖0.075% = 1.638)。不同糖浓度Y比较无统计学意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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