Assessment of Total Phenolic Compounds, Antioxidant Capacity, β-Carotene Bioaccessibility, HMF Formation, and Color Degradation Kinetics in Pumpkin Pestils

Azime ÖZKAN KARABACAK
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引用次数: 1

Abstract

Pestil, often known as fruit leather, is one of the most significant traditional foods manufactured and consumed throughout Türkiye. Due to its practical consumption, the availability of numerous nutrients, and the ability to meet energy requirements, pestil is recognized as a snack food. The aim of this study was to evaluate the bioaccessibility of total phenolic compounds (TPC), antioxidant capacity (AOC), and β-carotene in pumpkin pestils dried by hot air drying (HAD), vacuum drying (VCD), and microwave drying (MD) methods using an in vitro digestion model. Additionally, 5-hydroxymethylfurfural (HMF) formation and color degradation of pestils were evaluated. Changes in TPC and AOC were determined using spectrophotometric methods, whereas the detections of β-carotene and HMF were carried out with high performance liquid chromatography–photodiode array detector (HPLC–PDA). Significantly higher TPC (10.99–105.70%) and AOC (15.30–118.58%, 21.88–401.04% and 89.28–482.14%, in CUPRAC, FRAP, and DPPH assays, respectively) values were observed after drying (p<0.05). Moreover, it was observed that there were statistically significant increases in TPC and AOC values after digestion for all pumpkin pestils compared to undigested samples (p<0.05). Drying process resulted in lower β-carotene content (between 32.15–61.11%) in pumpkin pestils; however, it increased the percentage of bioaccessible β-carotene (max 62.16%) in the pestil samples. Compared to HD and VCD techniques, pumpkin pestils dried with MD exhibited significantly higher TPC, AOC and β-carotene content (p<0.05). All of the pumpkin pestils except those dried by MD at 180 W contain HMF below the Turkish Standards Institute legal limit of 50 mg/kg. L* value of pestils were described adequately to the zero- and first-order kinetic models while a* and b* values were only fitted to zero-order model. In conclusion, the findings obtained in this study pointed out that drying processes (especially by MD method) increased the bioaccessibility of TPC, AOC, and β-carotene.
南瓜瘟病病中总酚类化合物、抗氧化能力、β-胡萝卜素生物可及性、HMF形成和颜色降解动力学的评估
疫皮,通常被称为果皮,是整个挪威生产和消费的最重要的传统食品之一。由于其实际消耗,大量营养物质的可用性,以及满足能量需求的能力,鼠疫被公认为休闲食品。采用体外消化模型,研究了热风干燥(HAD)、真空干燥(VCD)和微波干燥(MD)三种干燥方法对南瓜皮中总酚类化合物(TPC)、抗氧化能力(AOC)和β-胡萝卜素的生物可及性。此外,还评价了5-羟甲基糠醛(HMF)的形成和颜色降解。采用分光光度法测定TPC和AOC的变化,采用高效液相色谱-光电二极管阵列检测器(HPLC-PDA)检测β-胡萝卜素和HMF。干燥后的TPC(10.99 ~ 105.70%)和AOC(15.30 ~ 118.58%, 21.88 ~ 401.04%, 89.28 ~ 482.14%)显著提高(p<0.05)。与未消化样品相比,消化后各南瓜病斑的TPC和AOC值均显著升高(p<0.05)。干燥处理导致南瓜枯萎病中β-胡萝卜素含量降低(32.15 ~ 61.11%);但可提高鼠疫菌样品中生物可及性β-胡萝卜素的含量,最高可达62.16%。与HD和VCD处理相比,MD处理的南瓜果实TPC、AOC和β-胡萝卜素含量显著高于HD和VCD处理(p<0.05)。除经MD在180瓦下干燥的南瓜外,所有南瓜的HMF含量均低于土耳其标准协会规定的50毫克/公斤的法定限量。L*值适合于零级和一阶动力学模型,而a*和b*值只适合于零级动力学模型。综上所述,本研究结果表明,干燥工艺(特别是MD法)提高了TPC、AOC和β-胡萝卜素的生物可及性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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