Effect of a Combination of Water Clover (Marsilea Crenata) Ethanol Extract and Moringa Leaf (Moringa oleifera) Ethanol Extract on Follicle and Oocyte Diameter of Goat: In Vitro Study

Septiawan Putranto, Sri Rahayu, G. Ciptadi, Siska Nanda Widhaningrum
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Abstract

Water clover (Marsilea crenata) and moringa leaves (Moringa oleifera) are types of herbal plants that are widely used in various alternative medicines. Pharmacologically, both have active compounds such as flavonoids which have cellular activities such as antioxidants and estrogenic. This study aims to evaluate the effect of a combination of water clover ethanol extract and Moringa leaf ethanol extract with different treatment groups with the same concentration on follicle and oocyte growth based on follicle diameter and oocyte diameter after six days of culture. This experimental study used 24 antral follicles (2.5-3.2 mm) which were isolated, and randomly divided into four treatment groups namely K (control) without supplementation of water clover ethanol extract and moringa leaf ethanol extract, group D1 with water clover ethanol extract, group D2 with supplementation of moringa leaf ethanol extract, and group D3 with supplementation of a combination of water clover ethanol extract and moringa leaf
水三叶草(Marsilea Crenata)乙醇提取物与辣木叶(Moringa oleifera)乙醇提取物联合使用对山羊卵泡和卵母细胞直径影响的体外研究
水三叶草(Marsilea crenata)和辣木叶(moringa oleifera)是一种被广泛用于各种替代药物的草药植物。从药理学上讲,两者都含有活性化合物,如类黄酮,它具有抗氧化剂和雌激素等细胞活性。本研究旨在以培养6 d后的卵泡直径和卵母细胞直径为指标,评价水三叶草乙醇提取物和辣木叶乙醇提取物在相同浓度下不同处理组对卵泡和卵母细胞生长的影响。本实验选取24个分离的2.5 ~ 3.2 mm的大鼠心房卵泡,随机分为不添加水三叶草乙醇提取物和辣木叶乙醇提取物的K(对照)组、添加水三叶草乙醇提取物的D1组、添加辣木叶乙醇提取物的D2组、添加水三叶草乙醇提取物和辣木叶联合添加的D3组
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