Enhancement of Phycocyanin Extraction from Dry Spirulina platensis Powder by Freezing-Thawing Pre-treatment

IF 1.4 4区管理学 Q2 ENGINEERING, MULTIDISCIPLINARY

International Journal of Technology Management Pub Date : 2023-06-28 DOI:10.14716/ijtech.v14i4.5169

E. Sulistiawati, R. Rochmadi, M. Hidayat, A. Budiman

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引用次数: 2

Abstract

. Phycocyanin (PC) is a bioactive compound that can function as an antioxidant, anti-inflammatory, immunomodulatory, and anti-cancer agent. It can act as a potential material in preventing COVID-19 and curing those suffering from it. Spirulina platensis (SP) is one of the microalgae rich in proteins and PC. This study aimed to determine the optimum PC extraction from SP, using distilled water as solvent through freezing-thawing pre-treatment. The variables set in the investigation were water content in SP before freezing (24.7-84.9 % wet basis), soaking time (0.25, 1, 2, and 6 hours), raw materials’ storage period (1 -13 months), freezing time (1-141 days), and the (solvent/biomass) ratio (20-440 mL/g). Spirulina platensis powder was soaked, frozen, thawed, and extracted in batch operation. The residue was extracted with the same solvent. The PC concentration in the filtrate was determined by measuring its absorbance using a spectrophotometer at wavelengths 615 and 652 nm. The experiment gave the optimum yield at a water content of 81.9% (wet basis), soaking time of 6 hours, freezing time of 1 day, and a solvent-to-biomass ratio of 100 mL/g. The optimum storage period of the raw material was one month. The phycocyanin IC 50 value of 1.485 mg/L.

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冻融预处理强化干螺旋藻粉中藻蓝蛋白的提取

．藻蓝蛋白(PC)是一种具有抗氧化、抗炎、免疫调节和抗癌作用的生物活性化合物。它可以作为预防COVID-19和治疗患者的潜在材料。螺旋藻(Spirulina platensis, SP)是一类富含蛋白质和PC的微藻。本研究以蒸馏水为溶剂，通过冻融预处理，确定从SP中提取PC的最佳工艺条件。研究变量为冷冻前SP含水量(24.7- 84.9%湿基)、浸泡时间(0.25、1、2和6小时)、原料储存期(1 -13个月)、冷冻时间(1-141天)和(溶剂/生物质)比(20-440 mL/g)。采用浸泡、冷冻、解冻、分批提取的方法对螺旋藻粉进行提取。用相同的溶剂提取残渣。用分光光度计测定滤液在615和652nm波长处的吸光度，测定滤液中PC的浓度。实验结果表明，最佳产率为含水量81.9%(湿基)，浸泡时间6小时，冷冻时间1天，溶剂比为100 mL/g。该原料的最佳贮存期为1个月。藻蓝蛋白ic50值为1.485 mg/L。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Technology Management 管理科学-工程：综合

CiteScore

2.70

自引率

3.60%

发文量

审稿时长

6-12 weeks

期刊介绍： The IJTM aims to provide a refereed and authoritative source of information in the field of managing with technology, and the management of engineering, science and technology. It seeks to establish channels of communication between government departments, technology executives in industry, commerce and related business, and academic experts in the field.