Patterns of lactate and sorbitol dehydrogenase gene expression during the development of interspecific Rasbora hybrids

Abstract

The expression of alleles encoding the enzymes lactate dehydrogenase (LDH; EC 1.1.1.27) and sorbitol dehydrogenase (SDH; EC 1.1.1.14) was investigated in the redtail rasbora (Rasbora borapetensis), the eyespot rasbora (R. dorsiocellata), and in their reciprocal hybrids, by acrylamide gel electrophoresis. The spatial and temporal expression of LDH and SDH isozymes in Rasbora is consistent with those reported for other teleosts. The asynchronous delay in appearance of isozymes of paternal and maternal LDH-C and SDH-A subunit composition is in keeping with observed effects of gene regulatory divergence where alleles of maternal origin interact more effectively with maternal cytoplasmic regulatory factors than do alleles of paternal origin.