Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge

IF 0.6 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Wisan Chuavong, Sirikwan Ponprateep, Pravech Ajawatanawong, Tipachai Vatanavicharn
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Abstract

Polyhydroxybutyrate (PHB) is a natural polymer accumulated in granules as intracellular energy storage in stress condition. The aim of this research was to isolate potential PHB producing bacteria from canned tuna wastewater activated sludge and cow dung. Five positive bacterial isolates were confi rmed the accumulation of PHB using lipophilic staining and TEM. To identify the bacterial species, the phylogenetic analyses inferred from 16S rDNA gene sequence demonstrated that the bacterial strains were closely related to Bacillus genera. The comparison of PHB production from bacterial isolates showed that SWU01 strain isolated from the activated sludge exhibited the highest yield of PHB production. The cultivation of SWU01 stain in optimization condition (modifi ed M9 medium supplemented with 2% sodium acetate and 0.5% yeast extract at pH 7 for 48 h) exhibited the yield of PHB up to 0.95 g/L or 7.5 compared with M9 medium supplemented with 2% glucose. Transmission electron micrograph showed high among of PHB in the granule of the bacterial cell. From all results, the bacterial strain SWU01, effi cient PHB producers, exhibited the potential for their utilization in commercial PHB production.
从活性污泥中分离的新型巨芽孢杆菌SWU01生产聚羟基丁酸酯(PHB)
聚羟基丁酸酯(PHB)是一种天然聚合物,在应激条件下呈颗粒状积聚,作为细胞内的能量储存。本研究的目的是从金枪鱼罐头废水、活性污泥和牛粪中分离出可能产生PHB的细菌。通过亲脂染色和透射电镜,证实5株阳性分离菌均有PHB积累。根据16S rDNA基因序列进行系统发育分析,菌株与芽孢杆菌属亲缘关系较近。结果表明,从活性污泥中分离出的SWU01菌株的PHB产量最高。在优化条件下(改良M9培养基中添加2%醋酸钠和0.5%酵母提取物,pH为7,培养48 h),与添加2%葡萄糖的M9培养基相比,PHB的产量可达0.95 g/L或7.5。透射电镜显示,细菌细胞颗粒中PHB含量较高。综上所述,菌株SWU01是高效的PHB产生菌,具有商业化生产PHB的潜力。
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来源期刊
Chiang Mai Journal of Science
Chiang Mai Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.00
自引率
25.00%
发文量
103
审稿时长
3 months
期刊介绍: The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.
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