CHARACTERIZATION OF BIO-ABSORBABLE AND BIOMIMETIC GRANULES PRODUCED FROM ANIMAL BONE BY THE HIGH VELOCITY ROTATION-CRUSHING AND DEMINERALIZING TECHNIQUE

Phosphorus Research Bulletin Pub Date : 2012-01-01 DOI:10.3363/PRB.26.65

T. Akazawa, M. Murata, J. Tazaki, J. Hino, Katsuo Nakamura, S. Yoshinari, Y. Tabata, T. Hanawa, M. Takahata, N. Iwasaki, Manabu Ito, Tetsuya Ohmori, Hidekazu Yamachika, M. Kikuchi

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引用次数: 1

Abstract

granules originated from animal bone were easily prepared by using high velocity rotation-crushing apparatuses. Frozen bovine bone, porcine bone and human femoral bone were crushed together with saline ice for 1 min under 12,000 rpm-rotation number of a stainless-blade in a stainless vessel or a ZrO 2 -blade in a ZrO 2 vessel. The crushed granules exhibited granular sizes of 0.5-2 mm, which were efficient for regeneration of alveolar bone. They were dissolved at 293K under 500 rpm for 20 min in 2.0% HNO 3 aqueous solutions to obtain completely demineralized bone matrix (DBM) granules. The DBM granules were implanted into the subcutaneous tissue of the back region in nude mice. At 4-6 weeks after the implantation, osteoinduction or cartilage-induction as well as bio-absorption were recognized on the surface layers of both DBM granules. The DBM granules may be applied as new biomimetic materials for bone-regeneration therapy and physiological activation substances for biochemistry.

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高速旋转破碎脱矿技术制备动物骨可吸收仿生颗粒的研究

采用高速旋转粉碎装置制备动物骨源颗粒是一种简便易行的方法。将冷冻的牛骨、猪骨和人股骨与盐水冰一起粉碎，在不锈钢容器中的不锈钢刀片或ZrO - 2容器中的ZrO - 2刀片旋转12000转的条件下，粉碎1分钟。粉碎后的颗粒大小为0.5 ~ 2 mm，有利于牙槽骨的再生。在2.0% hno3水溶液中，在293K下，500 rpm下溶解20 min，得到完全脱矿的骨基质(DBM)颗粒。将DBM颗粒植入裸鼠背部皮下组织。植入后4-6周，两种DBM颗粒表面均出现骨诱导或软骨诱导及生物吸收。DBM颗粒可作为骨再生治疗的新型仿生材料和生物化学的生理活化物质。

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Phosphorus Research Bulletin

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