In vitro and in vivo toxicological evaluation of green synthesised anatase TiO2 nanoforms

Q2 Engineering

Materials Research Innovations Pub Date : 2022-07-05 DOI:10.1080/14328917.2022.2094629

P. Shubha, K. Namratha, M. Gowda, Mb. Nayan, H. Manjunatha, K. Byrappa

{"title":"In vitro and in vivo toxicological evaluation of green synthesised anatase TiO2 nanoforms","authors":"P. Shubha, K. Namratha, M. Gowda, Mb. Nayan, H. Manjunatha, K. Byrappa","doi":"10.1080/14328917.2022.2094629","DOIUrl":null,"url":null,"abstract":"ABSTRACT The present study aims at the evaluation of in vitro and in vivo toxicities of green TiO2 nanoparticles (GS-TiO2) that are biosynthesised using hydroalcoholic extract of Ocimum sanctum leaves using microwave energy. Commercially available TiO2 NPs (CS-TiO2) were used for comparison. In vitro hemotoxicity was evaluated using haemolysis assay, and viability of fibroblast cells using WST-8 assay. In vivo toxicity evaluation was performed using the silkworm model. Haemolysis assay showed that GS-TiO2 caused acceptable haemolysis of 2.3% and CS-TiO2 showed higher haemolysis of 4.4%. As per WST-8 assay, viability of fibroblast cells was better in groups treated with GS-TiO2 with more than 50% survival. Silkworm growth parameters were significantly improved in GS-TiO2-treated batches. At the biomolecular level, changes in haemolymph protein banding patterns indicated strong growth promoting potential of GS-TiO2. Considering the salient findings derived from toxicity results, we propose that GS-TiO2 shall be potent NPs for various biomedical applications.","PeriodicalId":18235,"journal":{"name":"Materials Research Innovations","volume":"33 1","pages":"138 - 144"},"PeriodicalIF":0.0000,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materials Research Innovations","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/14328917.2022.2094629","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Engineering","Score":null,"Total":0}

引用次数: 1

Abstract

ABSTRACT The present study aims at the evaluation of in vitro and in vivo toxicities of green TiO2 nanoparticles (GS-TiO2) that are biosynthesised using hydroalcoholic extract of Ocimum sanctum leaves using microwave energy. Commercially available TiO2 NPs (CS-TiO2) were used for comparison. In vitro hemotoxicity was evaluated using haemolysis assay, and viability of fibroblast cells using WST-8 assay. In vivo toxicity evaluation was performed using the silkworm model. Haemolysis assay showed that GS-TiO2 caused acceptable haemolysis of 2.3% and CS-TiO2 showed higher haemolysis of 4.4%. As per WST-8 assay, viability of fibroblast cells was better in groups treated with GS-TiO2 with more than 50% survival. Silkworm growth parameters were significantly improved in GS-TiO2-treated batches. At the biomolecular level, changes in haemolymph protein banding patterns indicated strong growth promoting potential of GS-TiO2. Considering the salient findings derived from toxicity results, we propose that GS-TiO2 shall be potent NPs for various biomedical applications.

查看原文本刊更多论文

绿色合成锐钛矿TiO2纳米形态的体内外毒理学评价

摘要:本研究旨在评价绿色二氧化钛纳米粒子(GS-TiO2)的体外和体内毒性，该纳米粒子是利用微波能量生物合成的。采用市售的TiO2 NPs (CS-TiO2)进行比较。采用溶血法评估体外血液毒性，采用WST-8法评估成纤维细胞活力。采用家蚕模型进行体内毒性评价。溶血实验表明，GS-TiO2溶血率为2.3%，CS-TiO2溶血率较高，为4.4%。WST-8实验结果显示，GS-TiO2处理组成纤维细胞存活率较高，存活率超过50%。gs - tio2处理批次的家蚕生长参数显著改善。在生物分子水平上，血淋巴蛋白带模式的变化表明GS-TiO2具有很强的促生长潜力。考虑到从毒性结果中得出的显著发现，我们建议GS-TiO2应该是各种生物医学应用的有效NPs。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Materials Research Innovations 工程技术-材料科学：综合

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

2.8 months

期刊介绍： Materials Research Innovations covers all areas of materials research with a particular interest in synthesis, processing, and properties from the nanoscale to the microscale to the bulk. Coverage includes all classes of material – ceramics, metals, and polymers; semiconductors and other functional materials; organic and inorganic materials – alone or in combination as composites. Innovation in composition and processing to impart special properties to bulk materials and coatings, and for innovative applications in technology, represents a strong focus. The journal attempts to balance enduring themes of science and engineering with the innovation provided by such areas of research activity.