Genetic Diversity among Single, Double, And Triple Cross Hybrids in Beit Alpha Cucumber (Cucumis sativus)

Jordan Journal of Agricultural Sciences Pub Date : 2022-03-01 DOI:10.35516/jjas.v18i1.103

Adel M. Altamari, M. Akash, M. Kasrawi

{"title":"Genetic Diversity among Single, Double, And Triple Cross Hybrids in Beit Alpha Cucumber (Cucumis sativus)","authors":"Adel M. Altamari, M. Akash, M. Kasrawi","doi":"10.35516/jjas.v18i1.103","DOIUrl":null,"url":null,"abstract":"Genetic variation was investigated in fifteen single, eighteen three-way, and nine double hybrid crosses and their parents along with nine elite commercial cucumber hybrids. Six lines (NS5, NS9, NS17, NS29, NS31, and NS33) were used to develop these cross hybrids. Based on AFLP and SSR analysis, the studied cucumber genotypes have a narrow genetic base. The results also showed genetic differentiation compared to commercial varieties. Out of 1352 bands produced from 12 AFLP primer pairs, only 47 were polymorphic. Similarly, out of 26 SSR primers, only one primer was polymorphic. The highest heterozygosity index (0.6044), polymorphic information content value (0.5392), and marker Index (0.6044) were observed with M-CTT_E-AAG AFLP primer combination, while the highest effective multiplex ratio (2.8246) and resolving power (4.8421) were observed with M-CTC_E-AGC AFLP primer combination. Genetic distance estimation revealed clear distinctiveness of the studied genotypes with the control varieties. The cluster analysis by UPGMA showed two main clusters covering 84% of the studied genotypes grouped. This research shows that double and three-way hybrid crosses can be used in cucumber hybrid breeding.","PeriodicalId":14707,"journal":{"name":"Jordan Journal of Agricultural Sciences","volume":"27 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jordan Journal of Agricultural Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35516/jjas.v18i1.103","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Genetic variation was investigated in fifteen single, eighteen three-way, and nine double hybrid crosses and their parents along with nine elite commercial cucumber hybrids. Six lines (NS5, NS9, NS17, NS29, NS31, and NS33) were used to develop these cross hybrids. Based on AFLP and SSR analysis, the studied cucumber genotypes have a narrow genetic base. The results also showed genetic differentiation compared to commercial varieties. Out of 1352 bands produced from 12 AFLP primer pairs, only 47 were polymorphic. Similarly, out of 26 SSR primers, only one primer was polymorphic. The highest heterozygosity index (0.6044), polymorphic information content value (0.5392), and marker Index (0.6044) were observed with M-CTT_E-AAG AFLP primer combination, while the highest effective multiplex ratio (2.8246) and resolving power (4.8421) were observed with M-CTC_E-AGC AFLP primer combination. Genetic distance estimation revealed clear distinctiveness of the studied genotypes with the control varieties. The cluster analysis by UPGMA showed two main clusters covering 84% of the studied genotypes grouped. This research shows that double and three-way hybrid crosses can be used in cucumber hybrid breeding.

查看原文本刊更多论文

贝特阿尔法黄瓜单、双、三交杂种的遗传多样性

对15个单杂交、18个三杂交和9个双杂交组合及其亲本和9个黄瓜优质商品杂交种进行了遗传变异研究。选用NS5、NS9、NS17、NS29、NS31和NS33 6个系进行杂交。基于AFLP和SSR分析，所研究的黄瓜基因型具有狭窄的遗传基础。结果还显示了与商品品种相比的遗传分化。从12对AFLP引物中得到的1352个条带中，只有47个是多态性的。同样，在26个SSR引物中，只有一个引物是多态性的。M-CTT_E-AAG AFLP引物组合的杂合度指数最高(0.6044)，多态性信息含量值最高(0.5392)，标记指数最高(0.6044)，M-CTC_E-AGC AFLP引物组合的有效多重倍率最高(2.8246)，分辨能力最高(4.8421)。遗传距离估计表明，所研究的基因型与对照品种有明显的差异。UPGMA聚类分析显示，两个主要聚类覆盖了84%的研究基因型。本研究表明，双杂交和三元杂交可用于黄瓜杂交育种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Jordan Journal of Agricultural Sciences

自引率

0.00%

发文量