3':5'-Cyclic nucleotides: two sodium salts of cdTMP.

IF 0.8 4区 化学
K. Ślepokura
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引用次数: 1

Abstract

3':5'-Cyclic nucleotides play an outstanding role in signal transduction at the cellular level but, in spite of comprehensive knowledge of the biological role of cyclic nucleotides, their structures are not established fully. Two hydrated sodium salts of thymidine 3':5'-cyclic phosphate (cdTMP, C10H12N2O7P), namely sodium thymidine 3':5'-cyclic phosphate heptahydrate, Na(+)·C10H12N2O7P(-)·7H2O or Na(cdTMP)·7H2O, (I), and sodium thymidine 3':5'-cyclic phosphate 3.7-hydrate, Na(+)·C10H12N2O7P(-)·3.7H2O or Na(cdTMP)·3.7H2O, (II), have been obtained in crystalline form and structurally characterized, revealing one nucleotide in the asymmetric unit of (I) and eight different nucleotides in (II). All the cyclic nucleotide anions adopt a similar conformation with regard to nucleobase orientation, sugar conformation and 1,3,2-dioxaphosphorinane ring puckering. In (I), no direct inter-nucleotide hydrogen bonds are present, and adjacent nucleotide anions interact via water-mediated and Na(+)-mediated contacts. In contrast, in (II), direct thymine-phosphate N-H...O inter-nucleotide hydrogen bonds occur and these are assisted by numerous inter-nucleotide C-H...O contacts, giving rise to the self-assembly of cdTMP(-) anions into three different ribbons. Two of these three ribbons run in the same direction, while the third is antiparallel.
3':5'-环核苷酸:cdTMP的两种钠盐。
3':5'-环核苷酸在细胞水平的信号转导中发挥着突出的作用,但是,尽管对环核苷酸的生物学作用有全面的了解,但其结构尚未完全建立。制备了两种胸腺嘧啶3′:5′-环磷酸(cdTMP, C10H12N2O7P)水合钠盐,即胸腺嘧啶3′:5′-环磷酸七水合钠(Na(+)·C10H12N2O7P(-)·7H2O或Na(cdTMP)·7H2O, (I)和胸腺嘧啶3′:5′-环磷酸3.7-水合钠(Na(+)·C10H12N2O7P(-)·3.7H2O或Na(cdTMP)·3.7H2O, (II),并对其结构进行了表征。在(I)的不对称单元中发现了一个核苷酸,在(II)中发现了八个不同的核苷酸。所有的环核苷酸阴离子在核碱基取向、糖构象和1,3,2-二磷烷环褶皱方面都采用相似的构象。在(I)中,不存在直接的核苷酸间氢键,相邻的核苷酸阴离子通过水介导和Na(+)介导的接触相互作用。相反,在(II)中,直接胸腺嘧啶-磷酸N-H…O核苷酸间的氢键发生,这些是由许多核苷酸间的C-H…O接触,引起cdTMP(-)阴离子自组装成三个不同的带。这三条缎带中的两条在同一方向上运行,而第三条是反平行的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
自引率
12.50%
发文量
0
审稿时长
1 months
期刊介绍: Acta Crystallographica Section C: Structural Chemistry is continuing its transition to a journal that publishes exciting science with structural content, in particular, important results relating to the chemical sciences. Section C is the journal of choice for the rapid publication of articles that highlight interesting research facilitated by the determination, calculation or analysis of structures of any type, other than macromolecular structures. Articles that emphasize the science and the outcomes that were enabled by the study are particularly welcomed. Authors are encouraged to include mainstream science in their papers, thereby producing manuscripts that are substantial scientific well-rounded contributions that appeal to a broad community of readers and increase the profile of the authors.
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