Multiphoton Light-sheet Microscopy at Optimal Pulse Frequency for Fast In Vivo Imaging

Frontiers in optics. Annual Meeting of the Optical Society of America Pub Date : 2020-09-14 DOI:10.1364/FIO.2020.FW5E.5

V. Maioli, Antoine Boniface, P. Mahou, Júlia Ferrer Ortas, L. Abdeladim, E. Beaurepaire, Willy Supatto

引用次数: 1

Abstract

Light-sheet illumination improves multiphoton imaging speed. By optimizing laser pulse frequency using the quantification of photodamage in embryos, we demonstrate an order-of-magnitude signal increase in live two-photon light-sheet imaging.

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最佳脉冲频率下的多光子光片显微镜快速体内成像

光片照明提高了多光子成像速度。通过利用胚胎光损伤的量化优化激光脉冲频率，我们证明了活双光子光片成像的数量级信号增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Frontiers in optics. Annual Meeting of the Optical Society of America

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