Protective influence of the zinc complex of 1-butylvioluric acid on the development of the liver tissue culture in the presence of convulsive poisons

Abstract

Introduction. Currently, the actual problem is the search for substances that can be used as drugs for the pharmacological correction of the effects of cytostatic damage by convulsive poisons. It is known that the liver is the first organ to encounter any foreign molecule carried by the portal bloodstream, and it is the most damaged. The aim of this work was to study the effect of the zinc complex of 1-butylvioluric acid on the development of an organotypic culture of rat liver tissue after poisoning with 2-(dimethylaminomethyl)phenyl ester of dimethylcarbamic acid hydrochloride. Material and methods. White 3-month-old male Wistar rats were used in the experiment. To study the effects of the test substances, liver tissue was selected and the method of organotypic cultivation was applied. Poisoning with convulsive poisons was modeled using 2-(dimethylaminomethyl)phenyl ester of dimethylcarbamic acid hydrochloride and the zinc complex of 1-butylvioluric acid was used as a drug for pharmacological correction of the effects of liver tissue damage. Results. The data obtained indicate that under the action of 2-(dimethylaminomethyl) phenyl ester of dimethylcarbamic acid hydrochloride, which simulates the effect of convulsive poisons on liver cells, their cell proliferation is inhibited. It was also found that the zinc complex of 1-butylvioluric acid eliminates the inhibitory effect of 2-(dimethylaminomethyl)phenyl ester of dimethylcarbamic acid hydrochloride on the proliferation of liver tissue culture. Research limitations. The study was performed on a culture of rat liver cells; to extrapolate data to the whole organism, it is necessary to take into account the data of toxicodynamics and toxicokinetics. Conclusion. Thus, the data obtained in the experiments prove the effectiveness of the use of the zinc complex of 1-butylvioluric acid as a drug for the pharmacological correction of the consequences of exposure to convulsive poisons, and also create a basis for its further study.