Evaluation of hepatoprotective potential of ethanolic extract of Ixora pavetta against isoniazid and rifampicin induced hepatotoxicity in rats

Drug Invention Today Pub Date : 2013-09-01 DOI:10.1016/j.dit.2013.06.007

Gangavaram Jyothi Reddy , Vara Prasanth Reddy , Mungura Sreepavani , Cuddapa Rajaram , Sadhu Nelson Kumar , Rupesh S. Kanhere

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引用次数: 13

Abstract

Objective

The aim of present study was to demonstrate the hepatoprotective effect of ethanolic extract of Ixora pavetta (EEIP) against isoniazid and rifampicin induced hepatotoxicity in rats.

Method

Rats were divided into five groups each group containing 6 animals. All rats were treated with INH + RIF in saline water at the dose of 100 mg/kg b.w., p.o. to the experimental animals for 21 days. Group I served as control administered with distilled water. In order to study the effect of EEIP in rats, 200 mg/kg b.w. and 400 mg/kg b.w. of extracts were administered to the rats in group IV and V by oral route. Silymarin (2.5 mg/kg b.w., p.o.) was used as a standard drug in this study. After the course of treatment the animals were sacrificed and blood and liver samples were collected for biochemical and histopathological studies respectively.

Results

Biochemical parametric evaluation of both the standard and EEIP (200 mg and 400 mg/kg b. wt) treated rats showed significant decrease in SGOT, SGPT, ALP, Total Bilirubin, Direct Bilirubin, and Total cholesterol. And the level of Total protein was significantly increased in both standard and EEIP treated rats when compared to toxic control group rats. The changes in biochemical parameters were supported by histological profile.

Conclusion

It is concluded that the ethanolic extract of Ixora pavetta protects against rifampicin and Isoniazid-induced oxidative liver injury in rats.

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野藿香醇提物抗异烟肼和利福平所致大鼠肝毒性的保护作用评价

目的研究野藿香醇提物(eiip)对异烟肼和利福平所致大鼠肝毒性的保护作用。方法随机分为5组，每组6只。所有大鼠均以100mg /kg b.w.的盐水给药INH + RIF 21 d。第一组为对照组，给予蒸馏水。为了研究EEIP对大鼠的影响，IV组和V组大鼠分别口服提取物200 mg/kg b.w.和400 mg/kg b.w.。以水飞蓟素(2.5 mg/kg b.w.， p.o.)为标准药物。疗程结束后处死动物，取血液和肝脏标本分别进行生化和组织病理学研究。结果经生化参数评价，大鼠血清SGOT、SGPT、ALP、总胆红素、直接胆红素和总胆固醇均显著降低。与中毒对照组相比，标准组和EEIP组大鼠总蛋白水平均显著升高。组织学特征支持了生化参数的变化。结论黄花菜醇提物对利福平和异烟肼所致大鼠氧化性肝损伤具有一定的保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Drug Invention Today

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