Effects of Butylated HydroxylToluene and Vitamin E on Cadmium-Lead toxicity on the liver of rats

H. Kadiri, Blessing Idholo
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Abstract

Abstract This study determined the effect of lead-cadmium toxicity in the liver of albino rats. 56 rats were divided into 7 groups of 8 rats each and were treated as follows : Group A Control (1 ml of diluent (corn oil); Group B (15mg/kg BW cadmium); Group C(50mg/kg BW lead); Group D (15mg/kg BW Cadmium and 50mg/kg BW lead); Group E (15mg/kg BW cadmium, 50mg/kg BW lead and 23mg/kg BW vitamin E);Group F (15mg/kg BW cadmium, 50mg/kg BW lead and 25mg/kg BW Butylated HydroxylToluene) and Group G (15 mg/kg BW Cd+50mg/kg BW lead+25mg/kg BW (BHT + vitamin E). Rats were sacrificed on the 29th and 56th day in sub-acute and chronic exposure respectively. Liver function, oxidative stress markers and histological examination were carried out. Results indicate that rats exposed to cadmium and lead suffered liver damage due to the significant (P<0.05) increase in Alanine Transferase, Aspartate transferase and Alkaline phosphatase, and significant decrease (P<0.05) in Total Protein and Albumin in the serum. Malonaldehyde (MDA) levels were also significantly increased (P<0.05) with a concomitant decrease in Superoxide dismutase, catalase and Glutathione peroxidase activities in both the chronic and acute exposure phase. Vitamin E and BHT singly had little ameliorative effect on the liver; however, a combination of these antioxidants reduced the enzymes levels significantly. Treatment with the antioxidants (Vitimin E and BHT) both singly and combined was also able to ameliorate the effects of the oxidative stress induced by the metals as was further confirmed by the histopathological reports.
丁基羟基甲苯和维生素E对大鼠肝脏镉铅毒性的影响
摘要本研究探讨了铅镉对白化病大鼠肝脏的毒性作用。将56只大鼠分为7组,每组8只大鼠,按如下方法处理:A组对照组(玉米油稀剂)1 ml;B组(镉15mg/kg BW);C组(铅50mg/kg BW);D组(镉15mg/kg体重、铅50mg/kg体重);E组(15mg/kg BW镉、50mg/kg BW铅和23mg/kg BW维生素E)、F组(15mg/kg BW镉、50mg/kg BW铅和25mg/kg BW丁基羟基甲苯)和G组(15mg/kg BW Cd+50mg/kg BW铅+25mg/kg BW (BHT +维生素E))分别于亚急性和慢性暴露第29天和第56天处死大鼠。进行肝功能、氧化应激指标及组织学检查。结果表明,镉、铅暴露大鼠血清中丙氨酸转移酶、天冬氨酸转移酶和碱性磷酸酶显著升高(P<0.05),总蛋白和白蛋白显著降低(P<0.05)。慢性和急性暴露期丙二醛(MDA)水平显著升高(P<0.05),同时超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性降低。单独使用维生素E和BHT对肝脏的改善作用不大;然而,这些抗氧化剂的组合显著降低了酶的水平。组织病理学报告进一步证实,抗氧化剂(维生素E和BHT)单独或联合治疗也能够改善金属诱导的氧化应激的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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