Growth Promotion Of Chick Pea By Native Phosphate Solubilizing And Auxin Producing Bacteria

Sadaf Shahab, N. Ahmed
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Abstract

Solubilization of insoluble organic phosphate has been the focus of many studies as it increases the availability of phosphorus to vegetation and improves plant growth. The aim of this study was to study those bacterial strains which were positive for phosphate solubilization in plate assay as well as in liquid media. A total of 70 metal solubilizing indigenous bacterial strain were isolated. Ten efficient phosphate solubilizing bacterial stains were investigated for phosphate solubilization in liquid media. Growth substances produced by these ten bacterial stains were determined via bioassay. Three bacterial strainsCMG851,CMG857 and CMG860 which found positive to auxin production were further investigated for indole acetic acid and indole butyric acid production .It was found that Indole acetic acid , indole butyric acid were produced by these bacterial strains in varying concentration with and with out the addition of tryptophan. These bacterial strains showed stimulatory effects on the growth of root and shoot elongation of chick pea. Three promising bacterial strains CMG854, CMG857 and CMG860 were investigated to establish the effect on plant growth.
天然增磷产生长素菌对鹰嘴豆生长的促进作用
不溶性有机磷的增溶提高了植物对磷的利用率,促进了植物的生长,已成为许多研究的焦点。本研究的目的是研究在平板试验和液体培养基中对磷酸盐增溶呈阳性的菌株。共分离到70株金属增溶性本地菌株。研究了10种有效的磷酸盐增溶细菌染色剂在液体培养基中的磷酸盐增溶作用。通过生物测定法测定了这10种细菌染色剂产生的生长物质。对生长素生成阳性的3株菌株scmg851、CMG857和CMG860进行了生长素生成吲哚乙酸和吲哚丁酸的研究,发现在添加色氨酸和不添加色氨酸的情况下,这些菌株都能生成不同浓度的吲哚乙酸和吲哚丁酸。这些菌株对鹰嘴豆的根和茎伸长有促进作用。以CMG854、CMG857和CMG860为研究对象,研究其对植物生长的影响。
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