Experimental study of the effectiveness of ademethionine and inosine glycyl-cysteinyl-glutamate disodium in the treatment of dichloroethane-induced toxic hepatitis

Yu.V. Orlov, S. V. Gaiduk, V. G. Kuzmich
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Abstract

Introduction. Currently, in the search for drugs for the toxic liver damage treatment, considerable attention is paid to glutathione preparations and its metabolic precursors. There are separate reports in the literature on the hepatoprotective and antifibrotic effects of glutathione disulfide in viral liver lesions, which gives grounds for their additional study when exposed to hepatotropic substances. Material and methods. Performing a comparative assessment of the hepatoprotective effect of inosine glycyl-cysteinyl-glutamate disodium and ademetionine on the model of dichloroethane-induced toxic hepatitis in rats was carried out with the formation of the following experimental groups of animals: no treatment; with the introduction of physiological saline; with the introduction of inosine glycyl-cysteinyl-glutamate disodium; with the introduction of ademetionine, as well as a group of intact animals. The study drugs were administered intraperitoneally for 10 days. In the course of the study, biochemical indicators of cytolytic and cholestatic syndromes of liver damage were determined, the activity of its detoxification system and plastic function were assessed. Results. In groups that did not receive treatment, but received physiological saline, the lethality of experimental animals by the 20th day of observation was 40%, in the group treated with ademetionine – 10%. In the group treated with inosine glycyl-cysteinyl-glutamate disodium, no death of animals was observed. The use of inosine glycyl-cysteinyl-glutamate disodium led to a more significant decrease in the indicators of cytolytic and cholestatic syndromes, as well as normalization of the functional activity of the liver (restoration of the content of glutathione in the liver tissue) and its plastic function (restoration of the level of total blood serum protein and glycogen content in the liver parenchyma) to a greater extent than with ademetionine. limitation. The experimental study was carried out on outbred male rats weighing 180–220 g, kept under standard vivarium conditions in one cage, no more than 6 individuals, divided into groups by randomization with the exclusion of weakened and sick animals from the study. Conclusion. The study provided an experimental justification for further study of the hepatoprotective effect of inosine glycylcysteinylglutamate disodium in toxic liver damage.
腺苷蛋氨酸和肌苷甘酰半胱氨酸谷氨酸二钠治疗二氯乙烷所致中毒性肝炎疗效的实验研究
介绍。目前,在寻找治疗中毒性肝损伤的药物时,谷胱甘肽制剂及其代谢前体受到了相当大的关注。文献中有关于谷胱甘肽二硫醚对病毒性肝病变的肝保护和抗纤维化作用的单独报道,这为暴露于促肝物质时进行进一步研究提供了依据。材料和方法。比较评价肌苷-甘氨酸-半胱氨酸-谷氨酸二钠和腺苷对二氯乙烷所致中毒性肝炎模型大鼠的肝保护作用,实验动物分为以下实验组:不给药;配合生理盐水的引入;引入肌苷甘氨酸半胱氨酸谷氨酸二钠;随着腺苷的引入,以及一组完整的动物。研究药物经腹腔注射10天。在研究过程中,测定肝损伤的细胞溶解综合征和胆汁淤积综合征的生化指标,评估其解毒系统的活性和可塑性功能。结果。未给予生理盐水治疗组,观察第20天实验动物死亡率为40%,给予腺苷- 10%组。肌苷-甘氨酸-半胱氨酸-谷氨酸二钠组未见动物死亡。与使用腺苷甘氨酸半胱氨酸谷氨酸二钠相比,使用肌苷甘氨酸半胱氨酸谷氨酸二钠能更显著地降低细胞溶解综合征和胆汁淤积综合征的指标,并在更大程度上恢复肝脏的功能活性(恢复肝组织中谷胱甘肽的含量)及其可塑性功能(恢复肝实质中血清总蛋白和糖原含量的水平)。限制。实验研究选用体重180 ~ 220 g的近交系雄性大鼠,在标准饲养条件下,单笼饲养,不超过6只,随机分组,体弱多病排除。结论。本研究为进一步研究肌苷甘氨酸半胱氨酸谷氨酸二钠对中毒性肝损伤的保护作用提供了实验依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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