Inhibition of Lasiodiplodia pseudotheobromae Causing Fruit Rot Disease of Longan by Using Antagonistic Bacillus siamensis RFCD306

IF 0.6 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Aimalin Pipattanapuckdee, P. Seehanam, C. Tiyayon, Danai Boonyakait, K. Kunasakdakul, Salit Supakitthanakorn, O. Ruangwong
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引用次数: 0

Abstract

P ostharvest fruit rot disease of longan (Dimocarpus longan Lour.) caused by Lasiodiplodia pseudotheobromae is one of the most devastating problems for longan production in Thailand. To reduce the use of chemicals, the endophytic bacterium was isolated from healthy longan fruit for using as a biological control agent. The antagonistic bacterium Bacillus siamensis isolate RFCD306 which was identified based on morphological, biochemical and molecular characterization. It showed the antifungal effect with 94.40% of fungal growth inhibition which was conducted by a dual culture method. The efficacy of B. siamensis RFCD306 on the inhibition of fungal growth and conidial germination was observed under the scanning electron microscope (SEM) and revealed the abnormal germination of conidial and hyphal growth. Moreover, four antibiotic synthesis-related genes consisting of srfA, ituA, bacD and bymA were detected from B. siamensis RFCD306 by a polymerase chain reaction (PCR) technique. These genes might be involved in the antifungal activity of the antagonistic bacterium. Furthermore, the disease control efficiency of B. siamensis RFCD306 was evaluated on harvested longan fruits and showed that the bacterium decreased the incidence of fruit rot disease. Therefore, this study represented the effective B. siamensis RFCD306 for using as a biological agent in controlling postharvest fruit rot disease of longan.
拮抗暹罗芽孢杆菌RFCD306对龙眼果腐病的抑制作用
龙眼采后果腐病(Dimocarpus longan Lour.)是危害泰国龙眼生产的主要病害之一。为了减少化学药剂的使用,从健康的龙眼果实中分离出内生细菌作为生物防治剂。通过形态、生化和分子鉴定鉴定拮抗细菌暹罗芽孢杆菌(Bacillus siamensis) RFCD306。双培养法对真菌生长的抑制率为94.40%。在扫描电镜下观察了siamensis RFCD306对真菌生长和分生孢子萌发的抑制作用,发现孢子萌发和菌丝生长异常。利用聚合酶链反应(PCR)技术,从暹罗螺旋体RFCD306中检测到srfA、ituA、bacD和bymA 4个抗生素合成相关基因。这些基因可能与拮抗菌的抗真菌活性有关。此外,还在采收的龙眼果实上评价了siamensis RFCD306的防病效果,结果表明该细菌降低了果实腐烂病的发病率。因此,本研究表明,siamensis RFCD306可作为防治龙眼采后腐病的有效生物制剂。
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来源期刊
Chiang Mai Journal of Science
Chiang Mai Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.00
自引率
25.00%
发文量
103
审稿时长
3 months
期刊介绍: The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.
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