Surface Modification of Gentamicin-loaded Polylactic Acid (PLA) Microsphere Using Double Emulsion and Solvent Evaporation: Effect on Protein Adsorption and Drug Release Behaviour

IF 1.2 Q3 MULTIDISCIPLINARY SCIENCES

Journal of Physical Science Pub Date : 2019-02-15 DOI:10.21315/JPS2019.30.S1.7

N. Mamat, M. Jaafar, Z. Hamid

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引用次数: 7

Abstract

Polylactic acid (PLA) microsphere as a drug carrier has been extensively investigated for drug delivery systems. However, due to its limitation of surface hydrophobicity, surface modifications have been studied to improve its utilisation in tissue engineering applications. In the present study, PLA microsphere loaded with gentamicin (GENMS) was modified to enhance its hydrophilicity by surface treatment with additional of ethanol. Ethanol was applied as a co-treating medium in alkaline hydrolysis of NaOH to assist the hydroxide nucleophilic attack on the ester bond of PLA. Alkaline concentrations of NaOH and NaOH/ethanol was set at 0.15 M, 0.25 M and 0.35 M. After surface treatment, hydrophilicity of GENMS surface were improved significantly whereby contact angle reduced for about 23.1% and 26.8% for modification using NaOH and NaOH/ethanol, respectively, compared with the neat GENMS. Obvious surface roughness presented by NaOH/ethanol modification improved hydrophilicity of GENMS. As a result, protein adsorption on the GENMS surface treated by NaOH/ethanol were reduced than NaOH modification. Moreover, the highest encapsulation efficiency by NaOH/ethanol modification provided an advantage of co-treating by ethanol and has a greater drug release compared with NaOH modification.

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双乳液和溶剂蒸发法修饰庆大霉素聚乳酸微球的表面:对蛋白质吸附和药物释放行为的影响

聚乳酸(PLA)微球作为一种药物载体已被广泛研究用于给药系统。然而，由于其表面疏水性的局限性，人们研究了表面改性以提高其在组织工程中的应用。在本研究中，对负载庆大霉素(GENMS)的聚乳酸微球进行了表面改性，并添加了乙醇，以增强其亲水性。采用乙醇作为共处理介质，对NaOH进行碱性水解，以帮助氢氧化物亲核攻击PLA的酯键。NaOH和NaOH/乙醇的碱性浓度分别为0.15 M、0.25 M和0.35 M，表面处理后，GENMS表面亲水性明显提高，与纯GENMS相比，NaOH和NaOH/乙醇改性的GENMS表面接触角分别降低了23.1%和26.8%。NaOH/乙醇改性后的GENMS表面粗糙度明显，亲水性得到改善。结果表明，与NaOH修饰相比，NaOH/乙醇处理的GENMS表面对蛋白质的吸附减少。此外，氢氧化钠/乙醇修饰的包封效率最高，具有乙醇共处理的优势，并且与氢氧化钠修饰相比具有更大的药物释放。

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来源期刊

Journal of Physical Science Physics and Astronomy-Physics and Astronomy (all)

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： The aim of the journal is to disseminate latest scientific ideas and findings in the field of physical sciences among scientists in Malaysia and international regions. This journal is devoted to the publication of articles dealing with research works in Chemistry, Physics and Engineering. Review articles will also be considered. Manuscripts must be of scientific value and will be submitted to independent referees for review. Contributions must be written in English and must not have been published elsewhere.