Pathogenic detection of patients with pulmonary tuberculosis in Chongqing

Abstract

Objective To analyze the positive situation of pathogenic detection in patients with pulmonary tuberculosis (PTB) in Chongqing, in order to provide the basis for further improving the positive rate of pathogenic detection and timely diagnosis. Methods The qualified rate of sputum specimen, positive rates of smear, culture and molecular detection, duration and quality of molecular detection in TB laboratories from 25 counties in Chongqing were investigated. From the Tuberculosis Information Management System (TBMIS), the data of etiology positive rate of each county in Chongqing was exported. The etiology positive rates under different circumstances of various indicators were compared and analyzed. Results The etiological positive rate of group with qualified rate of sputum specimen ≥60.0% were higher than those with qualified rate of sputum specimen 0.05). The etiological positive rate of group with smear positive rate ≥5.0% were higher than those with smear positive rate 0.05). The etiological positive rate of group with culture positive rate ≥20.0% were higher than those with culture positive rate 0.05). The groups with positive rate of molecular detection more than or equal to 50.0%, duration of molecular detection more than or equal to 6 months and unsuccessful test rate less than 5.0%, of which the etiology positive rate were significantly higher than the groups with positive rate of molecular detection less than 50.0%, duration of molecular detection less than or equal to 1 month and unsuccessful test rate more than or equal to 5.0% respectively( P 0.05)。涂片阳性率>5.0%组的病原学阳性率髙于涂片阳性率 0.05)。培养阳性率≥20.0%组的病原学阳性率髙于培养阳性率 0.05)。分子检测阳性率≥50.0%组、分子检测开展时间≥6个月组及分子检测失败性率<5.0%组的区县, 其病原学阳性 率分别明显髙于分子检测阳性率<50.0%组、分子检测开展时间<1个月组及分子检测失败性率;≥5.0%组(均为 P <0.05)。 结论 开展分子生物学检测并保证检测质量, 提高分子检测阳性率, 是重庆市结核病实验室提高肺结核患者病原学阳 性率的主要措施。