Effect of copper oxide nanoparticles on gene expression of NMDA receptor

I. Sitnikov, D. Shaikhova, A. M. Amromina, M. Sutunkova, Y. Ryabova, Anastasiya V. Tazhigulova, V. Ruzakov
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Abstract

Introduction. Copper plays an important role in the metabolism of the brain, but particles of copper, in the nanometer range, exhibit neurotoxic properties and cause malfunctioning of brain cells. Material and methods. For 6 weeks, 3 times a week, the animals were injected with a suspension of NPs of copper oxide. The determination of the expression of the genes GRIN1, GRIN2a, and GRIN2b, encoding the proteins GluN1, GluN2a, and GluN2b, respectively, was carried out by real-time PCR with probes. Results. A statistically significant decrease in the expression level of genes encoding NMDA receptor proteins was determined when exposed to 0.5 mg/ml CuO nanoparticles (ΔCt(GRIN1) = 0.813; ΔCt(GRIN2A) = 3.477; ΔCt(GRIN2B) = 1.37) in comparison with control group (ΔCt(GRIN1) = 6.301; ΔCt(GRIN2A) = 7.823; ΔCt(GRIN2B) = 4.747). Conclusion. Evaluation of gene expression of the NMDA receptor may be present in a genetic marker to determine the toxic effect of copper oxide nanoparticles; however, further studies are needed, including behavioral tests to confirm the clinical manifestations of neurodegenerative disorders.
氧化铜纳米颗粒对NMDA受体基因表达的影响
介绍。铜在大脑的代谢中起着重要的作用,但纳米范围内的铜颗粒表现出神经毒性并导致脑细胞功能失调。材料和方法。连续6周,每周3次注射氧化铜NPs悬浮液。分别编码GluN1、GluN2a和GluN2b蛋白的基因GRIN1、GRIN2a和GRIN2b的表达通过探针实时荧光定量PCR检测。结果。暴露于0.5 mg/ml CuO纳米颗粒时,编码NMDA受体蛋白的基因表达水平有统计学意义的降低(ΔCt(GRIN1) = 0.813;ΔCt(GRIN2A) = 3.477;ΔCt(GRIN2B) = 1.37)与对照组(ΔCt(GRIN1) = 6.301;ΔCt(GRIN2A) = 7.823;ΔCt(GRIN2B) = 4.747)。结论。评估NMDA受体的基因表达可能存在于遗传标记中,以确定氧化铜纳米颗粒的毒性作用;然而,还需要进一步的研究,包括行为测试来确认神经退行性疾病的临床表现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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