Blind Deconvolution of Widefield Fluorescence Microscopic Data by Regularization of the Optical Transfer Function (OTF)

Abstract

With volumetric data from wide field fluorescence microscopy, many emerging questions in biological and biomedical research are being investigated. Data can be recorded with high temporal resolution while the specimen is only exposed to a low amount of photo toxicity. These advantages come at the cost of strong recording blur caused by the infinitely extended point spread function (PSF). For wide field microscopy, its magnitude only decays with the square of the distance to the focal point and consists of an airy bessel pattern which is intricate to describe in the spatial domain. However, the Fourier transform of the incoherent PSF (denoted as Optical Transfer Function (OTF)) is well localized and smooth. In this paper, we present a blind deconvolution method that improves results of state-of-the-art deconvolution methods on wide field data by exploiting the properties of the wide field OTF.