H. Radwan, A. Ateya, Eman A. Abo Elfad, Shimaa A. Sak, M. Fouda, R. Darwish, Adel E. El-Desoky
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引用次数: 0
Abstract
Melanocortin 4 receptor (MC4R), a protein derived from the MC4R gene, is involved in feed intake, metabolism control, and body weight regulation in humans. The purpose of this research was to explore MC4R polymorphisms alongside metabolic marker changes and their relationship with growth and carcass measurements in rabbits. Using synthetic line V (V-line) and Baladi Black rabbit breeds (60 rabbits per breed), blood samples were collected for DNA extraction and biochemical analysis. The polymerase chain reaction (PCR) product of MC4R (493 bp) revealed five nonsynonymous single nucleotide polymorphisms (SNPs; submitted to GenBank with accession numbers gb|MT832144|, gb|MT832145|, and gb|MT832146|). Four SNPs were characteristic of the V-line breed, and one was characteristic of the Baladi Black. For classification of the defined SNP-dependent groups within and between breeds, a discriminant analysis model correctly classified a percentage of cases with the following predictor variables: 90.8% for body weight at 5–14 weeks of age; 85% for feed consumption, daily feed intake, and feed conversion ratio; and 93.3% for carcass measurements (for which hind part weight, liver weight, and liver percentage were the best predictors in both breeds). There were significant differences between and within V-line and Baladi Black breeds in agreement with metabolic biochemical marker profiles and the defined SNPs. The identified SNPs in the MC4R gene and profile of the investigated metabolic biomarkers could be used as candidates and reference for the effective characterization of the two rabbit breeds. This study could therefore facilitate the introduction of markerassisted selection for growth performance characteristics in rabbits.
期刊介绍:
The South African Journal of Animal Science is an open access, peer-reviewed journal for
publication of original scientific articles and reviews in the field of animal science. The journal
publishes reports of research dealing with production of farmed animal species (cattle, sheep,
goats, pigs, horses, poultry and ostriches), as well as pertinent aspects of research on aquatic
and wildlife species. Disciplines covered nutrition, genetics, physiology, and production
systems. Systematic research on animal products, behaviour, and welfare are also invited.
Rigorous testing of well-specified hypotheses is expected.