Effect of Ethanol Concentration and Extraction Time with Microwave Assisted Extraction on Antioxidant Activity of Temulawak-Extract (Curcuma Xanthorrhiza.Roxb)

Abstract

Temulawak (Curcuma xanthorrhiza Roxb) is commonly used as traditional medicine. This study aimed at determining the  effect of ethanol concentration and of the duration of microwave-assisted extraction (MAE) on antioxidant activity of C. xanthorrhiza ethanol extract. C. xanthorrhiza rhizomes were extracted using 70%, 80% and 90% (v/v) ethanol for 5, 7 and 9 minutes. compared to control (without microwave extraction) and analyzed for curcumin content, total phenolic content (TPC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity (RSA). Increasing the ethanol concentration from 70% to 90% as solvent and increasing the extraction time from 0 to 7 minutes at 240 watt caused an increase in curcumin content, TPC, and DPPH RSA. Curcumin, TPC, and DPPH RSA of C. xahorrhiza extract using 90% ethanol solvent for 7 minutes were the largest compared to other treatments, namely 184 mg/g, 319,5 mg/g and 71.08%; respectively.