Influence des conditions de culture sur la respiration de Pseudomanas aeruginosa

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-12-23 DOI:10.1016/0926-6569(64)90005-7

Edgard Azoulay

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引用次数: 11

Abstract

1.
1. In the cells of Pseudomonas aeruginosa, the cytochrome oxidase studied by Yamanakaet al. (Pseudomonas cytochroom C-551 : nitrite O₂ oxidoreductase) is only induced in the presence of KNO₃. This enzyme is probably an important factor in the utilization of nitrates by this organism.
2.
2. A second cytochrome oxidase (cytochrome c: O₂ oxidoreductase, EC 1.9.3.1) is present in these cells. This enzyme is characterized by a strong absorption at 590 mμ and oxidizes the reduced cytochrome c of mammalian heart.
3.
3. When the cells are growing on glucose this enzyme appears in very small amounts. Apart from the fact that glucose induces a third type of respiratory system, which has not been determined but which is characterized by its insensibility to 10⁻⁴ M cyanide, glucose is a repressor of the formation of this enzyme.

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培养条件对铜绿假藻呼吸的影响

1.1. 在铜绿假单胞菌的细胞中，Yamanakaet al.研究的细胞色素氧化酶(Pseudomonas cytoroom C-551:亚硝酸盐O2氧化还原酶)仅在KNO3存在的情况下被诱导。这种酶可能是这种生物利用硝酸盐的一个重要因素。第二种细胞色素氧化酶(细胞色素c: O2氧化还原酶，EC 1.9.3.1)存在于这些细胞中。该酶的特点是在590 μ m下具有很强的吸收能力，并能氧化哺乳动物心脏中还原的细胞色素c。当细胞靠葡萄糖生长时，这种酶的数量非常少。除了葡萄糖能诱导第三种呼吸系统(尚未确定，但其特点是对10−4 M氰化物不敏感)外，葡萄糖是这种酶形成的抑制物。

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects

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