Combining the virulent Beauveria bassiana (Balsam) Vuillemin LCB289 and nematode strains to control pupae of Ceratitis capitata Wiedemann

IF 1.5 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocontrol Science and Technology Pub Date : 2023-03-23 DOI:10.1080/09583157.2023.2191300

C. Gava, B. Paranhos

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引用次数: 1

Abstract

ABSTRACT Entomopathogenic fungi (EF) and nematodes (EPN) can infect pupae of C. capitata Wiedemann in the soil, where they are protected from most control measures. This study evaluated infective juvenile (IJs) density to achieve high control efficiency, the residual effect of commercial strains of Heterorhabitidis bacteriophora (Poinar) and Steinernema carpocapsae (Weiser), and their combined application with Beauveria bassiana (Balsam) Vuillemin LCB289 on pupae mortality. Applying 106 IJs m−2 of both EPN species to columns with sandy Yellow Ultsol at laboratory temperature (24.0 ± 2°C) caused pupae mortality higher than 80%. Probit analysis showed that the curves for the EPN species had statistically similar slopes, with LC50 of 3.57 × 106 for H. bacteriophora and 2.12 × 106 for S. carpocapsae. The former showed a median potency 36% higher than H. bacteriophora. Microscope observations showed that infection by both species resulted in the melanization of pupae and no visual differences in the EPN growth in the cadavers. In a long-term experiment in greenhouse conditions (T = 26.5 ± 4.2°C; Ru = 62.7% ± 22.5), a single application of S. carpocapsae in columns with sandy Ultisol showed a residual effect of 21 days, while for H. bacteriophora it was lower than 15 days. Concomitant application of S. carpocapsae and the virulent EF strain B. bassiana LCB289 in columns with sandy soil in a greenhouse experiment in the same conditions (T = 26.5 ± 4.2°C; Ru = 68.5% ± 23.3) increased the median potency of the BCAs, resulting in a lower lethal concentration applied (LC50).

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球孢白僵菌LCB289与线虫菌株联合防治头角丝虫病蛹的研究

昆虫病原真菌(EF)和线虫(EPN)可以在土壤中感染C. capitata Wiedemann的蛹，在土壤中它们不受大多数控制措施的保护。本研究评估了侵染幼虫(IJs)的密度，以达到较高的防治效果，分析了商业菌株Heterorhabitidis bacteriophora (Poinar)和steinerma carpocapsae (Weiser)的残留效果，以及它们与球孢白僵菌(Balsam) Vuillemin LCB289联合施用对蛹死亡率的影响。在实验室温度(24.0±2°C)下，将这两种EPN的106 IJs m−2应用于沙质黄色Ultsol柱上，蛹死亡率高于80%。Probit分析表明，各EPN菌种的LC50斜率具有统计学上的相似性，分别为:H. bacteriophora和S. carpocapsae的LC50分别为3.57 × 106和2.12 × 106。前者的中位效价比嗜菌杆菌高36%。显微镜观察结果显示，两种感染均导致蛹黑化，尸体EPN生长无视觉差异。在温室条件下(T = 26.5±4.2°C;Ru = 62.7%±22.5)，砂质ultissol柱柱单次施用carpocapsae的残留效果为21 d，而对噬菌体H.的残留效果低于15 d。相同条件下，温室砂质土柱上同时施用稻瘟病菌(T = 26.5±4.2°C;Ru = 68.5%±23.3)增加了bca的中位效价，导致较低的致死浓度(LC50)。

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来源期刊

Biocontrol Science and Technology 生物-昆虫学

CiteScore

3.20

自引率

7.10%

发文量

审稿时长

4-8 weeks

期刊介绍： Biocontrol Science and Technology presents original research and reviews in the fields of biological pest, disease and weed control. The journal covers the following areas: Animal pest control by natural enemies Biocontrol of plant diseases Weed biocontrol ''Classical'' biocontrol Augmentative releases of natural enemies Quality control of beneficial organisms Microbial pesticides Properties of biocontrol agents, modes of actions and methods of application Physiology and behaviour of biocontrol agents and their interaction with hosts Pest and natural enemy dynamics, and simulation modelling Genetic improvement of natural enemies including genetic manipulation Natural enemy production, formulation, distribution and release methods Environmental impact studies Releases of selected and/or genetically manipulated organisms Safety testing The role of biocontrol methods in integrated crop protection Conservation and enhancement of natural enemy populations Effects of pesticides on biocontrol organisms Biocontrol legislation and policy, registration and commercialization.