Porous tubular scaffolds for tissue engineering structures of small diameter blood vessels

Abstract

A technology fo the formation of porous tubular polymer scaffolds (PTPS) made of polycaprolactone (PCL) with the necessary physical and mechanical characteristics (Young’s modulus 5.5 ± 1.1 MPa, breaking force 10.9 ± 1.6 N and maximum elongation 477 ± 38 %) has been developed. Samples obtained by electrospinning from 2 ml of a 10 % PCL solution in dichloromethane (voltage between electrodes 25 kV, solution delivery rate 4 ml/h, distance to the collector 100 mm, rotation speed of the substrate rod 1000 rpm) showed a minimum surgical permeability of 30.4 ± 1.5 ml/(cm2·min). It is proved that the proposed bioactive coating based on heparin and platelet lysate does not affect the surface structure and physical and mechanical characteristics of the scaffold. It has been shown in vitro that samples of modified PTPS do not exhibit cytotoxicity and hemolytic activity, and improvement of hemocompatible properties occurs due to a decrease in the number and degree of activation of adhered platelets.