A Phytochemical Study and Comparison of the Effect of Citrullus Colocynthis Extracts on Colon Cancer Cells Caco-2

Neda Mirzaei, M. Kolahi, Babak Mokhtari
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Abstract

Methods: The effective compounds of the air branches of Citrullus Colocynthis L. were studied using phytochemicals methods. In order to evaluate the cytotoxic and antioxidant characteristics of this plant, the ethanolic and n-hexane extracts of the plant were collected, using Soxhlet apparatus and maceration methods. The effects of these extracts on Caco2 colon cancer cells were evaluated using MTT tests based on the ability of living cells to convert tetrazolium salt to unsolvable formazan, NBT method to evaluate the reduction of ROS using aqueous nitrotrazole and based on a completely randomized design using SPSS software and Duncan test.
瓜蒌提取物对结肠癌细胞Caco-2作用的植物化学研究及比较
方法:采用植物化学方法对香瓜气枝的有效成分进行研究。为了评价该植物的细胞毒性和抗氧化特性,采用索氏仪和浸渍法收集了该植物的乙醇和正己烷提取物。采用MTT法(基于活细胞将四氮唑盐转化为不可溶甲醛的能力)、NBT法(基于水硝基曲唑还原ROS的能力)和基于完全随机设计(基于SPSS软件和Duncan检验)评估这些提取物对Caco2结肠癌细胞的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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