Agrobacterium-mediated transformation – method of genetic modification of Triticum aestivum L. plants

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2022-09-01 DOI:10.7124/feeo.v30.1466

A. Komisarenko, S. Mykhalska, V. M. Kurchii

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Abstract

Aim. Investigate the effect of Agrobacterium-mediated transformation in planta on seed tying and the frequency of transformation in winter wheat (Triticum aestivum L.). To analyze changes in the level of free L-proline (Pro) in transformed and control seedlings under normal / stress conditions and productivity indicators of biotechnological plants (T1) under normal growing conditions. Methods. Agrobacterium-mediated transformation in planta; PCR analysis, DNA electrophoresis; determination of seed tying frequency and transformation, Pro content, yield structure indicators. Results. Obtained transgenic wheat plants. The level of Pro in the tested variants under normal / stress conditions and indicators of T1 productivity of plants and their initial form under optimal water supply were studied. Conclusions. The susceptibility of the studied wheat genotypes to agrobacterial infection is shown. The frequency of seed tying after genetic transformation was 12.7 % and 5.4 % for plants of UK 106/19 and UK 171/19h, respectively. Transgenic seedlings had elevated levels of Pro. Complete incorporation of the vector construct was identified in 14 and 11 variants of genotypes UK 161/19 and UK 171/19h, respectively. Control and T1 biotechnological plants under normal growing conditions had similar yields.

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农杆菌介导转化——小麦植物基因改造方法

的目标。研究农杆菌介导的植物转化对冬小麦种子结扎及转化频率的影响。分析正常/胁迫条件下转化和对照幼苗游离l -脯氨酸(Pro)水平的变化以及正常生长条件下生物技术植物(T1)的生产力指标。方法。农杆菌介导的植物转化;PCR分析，DNA电泳;结扎频率和转化率、Pro含量、产量结构指标的测定。结果。获得转基因小麦植株。研究了正常/逆境条件下供试品种Pro的含量，以及最佳供水条件下植物T1生产力指标及其初始形态。结论。研究了小麦基因型对农杆菌感染的易感性。uk106 /19和uk171 /19h植株转化后结扎率分别为12.7%和5.4%。转基因幼苗的Pro水平升高。在基因型UK 161/19和UK 171/19h的14个和11个变体中分别鉴定了载体结构的完全整合。对照和T1生物技术植株在正常生长条件下产量相近。

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Faktori eksperimental'noi evolucii organizmiv

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