Structure and Function Changes of the Organ of Corti After Use of Sildenafil Citrate (VIAGRA®), in Adult Male Guinea Pigs

Q4 Medicine

Egyptian Journal of Ear, Nose, Throat and Allied Sciences Pub Date : 2022-01-01 DOI:10.21608/ejentas.2021.96751.1419

R. Zittoon

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引用次数: 0

Abstract

Background: Sildenafil citrate has been reported to induce temporary sensorineural hearing loss,tinnitus and dizziness. Objective: To evaluate the effects of sildenafil citrate on the guinea pigs cochlear structure and function using Otoacoustic Emissions (OAEs) and light microscopic H & E cochlear sections. Patients and Methods: This experimental study was conducted on 45 healthy adult male pigmented guinea pigs in Otolaryngology-Head and neck Surgery and Histology Departments in Suez Canal University, Ismailia-Egypt. Animals were randomly divided into three groups: Group 1 (n=15): control group. Group 2 (n=15): received the therapeutic dose of sildenafil citrate. Group 3 (n=15): received double the therapeutic dose of sildenafil citrate. Results: DPOAEs recorded from the three groups showed that the lowest amplitude was at 1006 kHz (10.4dB) and the highest at 6060 & 8084 kHz (43.9 & 42.1dB) respectively without statistically significant intergroup difference. In the H & E cochlear sections, there was disorganization and vacuolation of almost all cells of organ of Corti in group 2 and 3 compared to group 1. Lightly stained cytoplasm, karryorrhectic nuclei and pyknotic ones were also seen. These changes were more marked in group 3 which showed also some cellular loss in some sections. Conclusion: Therapeutic dose of sildenafil citrate affects the cellular structure of organ of corti without detectable effects in DPOAEs.

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成年雄性豚鼠使用枸橼酸西地那非(伟哥®)后Corti器官结构和功能的变化

背景:枸橼酸西地那非有报道可引起暂时性感音神经性听力丧失、耳鸣和头晕。目的:利用耳声发射(oae)和耳蜗光镜H & E切片观察枸橼酸西地那非对豚鼠耳蜗结构和功能的影响。患者与方法:本实验选取埃及伊斯梅利亚苏伊士运河大学耳鼻喉头颈外科和组织学部门的45只健康成年雄性色素豚鼠。将动物随机分为三组:第一组(n=15):对照组。组2 (n=15):给予治疗剂量的枸橼酸西地那非。第三组(n=15):给予双倍治疗剂量的枸橼酸西地那非。结果:三组dpoae记录显示，最低振幅在1006 kHz (10.4dB)，最高振幅在6060和8084 kHz(43.9和42.1dB)，组间差异无统计学意义。在h&e耳蜗切片中，与1组相比，2组和3组几乎所有的Corti器官细胞都出现了紊乱和空泡化。胞质染色较浅，核核呈核团状，核收缩。这些变化在第3组更为明显，在某些部分也显示出一些细胞损失。结论:枸橼酸西地那非治疗剂量对大鼠corti脏器细胞结构有影响，对dpoae无明显影响。

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来源期刊

Egyptian Journal of Ear, Nose, Throat and Allied Sciences Medicine-Otorhinolaryngology

CiteScore

0.20

自引率

0.00%

发文量

期刊介绍： Egyptian Journal of Ear, Nose, Throat and Allied Sciences aspires to play a national, regional and international role in the promotion of responsible and effective research in the field of Otolaryngology, Head and Neck Surgery in Egypt, Middle East and Africa. Mission To encourage and support research in Ear, Nose and Throat (ENT) field and interdisciplinary topics To implement high-quality editorial practices among Otolaryngologists To upgrade the ability and experience of local doctors in international publishing To offer professional publishing support to local researchers, creating a supportive network for career development To highlight ENT diseases and problems peculiar to our region To promote research in endemic, hereditary and infectious ENT problems related to our region To expose and study impact of ethnic, social, environmental and cultural issues on expression of different ENT diseases To organize common epidemiologic research of value to the region To provide resource to national and regional authorities about problems in the field of ENT and their implication on public health and resources To facilitate exchange of knowledge in our part of the world To expand activities with regional and international scientific societies.