Determination of the Anticarcinogenic Activity of 5-Hydroxymethyl-2-furfural Produced from Grape Must Under in vitro Conditions

Kübra Kelleci, Eda Gölebatmaz
{"title":"Determination of the Anticarcinogenic Activity of 5-Hydroxymethyl-2-furfural Produced from Grape Must Under in vitro Conditions","authors":"Kübra Kelleci, Eda Gölebatmaz","doi":"10.18596/jotcsa.1142274","DOIUrl":null,"url":null,"abstract":"Every year, millions of tons of food and beverage waste are thrown away unused around the world. The carbohydrates found in food waste create a raw material potential for the production of high value-added products that are used in energy, feed and pharmacology. One of these products, 5-Hydroxymethyl-2-furfural (5-HMF), is a by-product of simple dehydration of carbohydrates. It finds wide use in the field of pharmacy due to its anticancer, antifungal and antimicrobial activities. Many studies have stated that the sugar source with the highest conversion rate in 5-HMF production is fructose. For this reason, in this study, it was aimed to realize the production of 5-HMF in autoclave sterilization carried out under high temperature and pressure using grape must waste, which is known to have high fructose content, and determine the anticarcinogenic activity and cytotoxicity of the produced 5-HMF under in vitro conditions. In this study, it was determined that the medium containing DMSO increased the sugar conversion percentage, 5-HMF efficiency and selectivity in the waste grape must more than the medium containing only water. In the production of 5-HMF, the conversion of sugar in the medium saturated with salt, and the efficiency and selectivity of 5-HMF were determined as 97.04%, 68.61% and 70.82%, respectively, when DMSO organic solvent was used. In addition, it has been determined that 5-HMF produced from waste grape must has a toxic effect on both healthy cells and cancer cells and has anticancer properties.","PeriodicalId":17299,"journal":{"name":"Journal of the Turkish Chemical Society Section A: Chemistry","volume":"19 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Turkish Chemical Society Section A: Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18596/jotcsa.1142274","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Every year, millions of tons of food and beverage waste are thrown away unused around the world. The carbohydrates found in food waste create a raw material potential for the production of high value-added products that are used in energy, feed and pharmacology. One of these products, 5-Hydroxymethyl-2-furfural (5-HMF), is a by-product of simple dehydration of carbohydrates. It finds wide use in the field of pharmacy due to its anticancer, antifungal and antimicrobial activities. Many studies have stated that the sugar source with the highest conversion rate in 5-HMF production is fructose. For this reason, in this study, it was aimed to realize the production of 5-HMF in autoclave sterilization carried out under high temperature and pressure using grape must waste, which is known to have high fructose content, and determine the anticarcinogenic activity and cytotoxicity of the produced 5-HMF under in vitro conditions. In this study, it was determined that the medium containing DMSO increased the sugar conversion percentage, 5-HMF efficiency and selectivity in the waste grape must more than the medium containing only water. In the production of 5-HMF, the conversion of sugar in the medium saturated with salt, and the efficiency and selectivity of 5-HMF were determined as 97.04%, 68.61% and 70.82%, respectively, when DMSO organic solvent was used. In addition, it has been determined that 5-HMF produced from waste grape must has a toxic effect on both healthy cells and cancer cells and has anticancer properties.
葡萄叶5-羟甲基-2-糠醛体外抗癌活性的测定
每年,全世界有数百万吨未使用的食物和饮料垃圾被丢弃。在食物垃圾中发现的碳水化合物为生产用于能源、饲料和药理学的高附加值产品创造了潜在的原材料。其中一种产物,5-羟甲基-2-糠醛(5-HMF),是碳水化合物简单脱水的副产物。由于其具有抗癌、抗真菌和抗菌的作用,在医药领域得到了广泛的应用。许多研究表明,在5-羟甲基糠醛生产中转化率最高的糖源是果糖。因此,本研究旨在利用已知果糖含量高的葡萄渣在高温高压下进行高压灭菌,实现5-HMF的生产,并在体外条件下测定所生产的5-HMF的抗癌活性和细胞毒性。本研究确定含DMSO的培养基提高了废葡萄中糖的转化率、5-HMF的效率和选择性一定大于只含水的培养基。在制备5-羟甲基糠醛过程中,采用DMSO有机溶剂时,糖在饱和盐培养基中的转化率为97.04%,5-羟甲基糠醛的效率和选择性分别为68.61%和70.82%。此外,已经确定从废葡萄中产生的5-羟甲基糠醛对健康细胞和癌细胞都有毒性作用,并具有抗癌特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信