A rare cultivar of horse chestnut (Aesculus hippocastanum L. ˈBaumaniiˈ) in Slovakia: morphological, molecular and antioxidant analysis

Q4 Agricultural and Biological Sciences

Thaiszia Journal of Botany Pub Date : 2023-01-01 DOI:10.33542/tjb2023-1-02

K. Ražná, Ľ. Ďurišová, Matúš Kučka, Ľubomír Harenčár, E. Ivanišová, M. Habán, P. Hrubík

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引用次数: 0

Abstract

Aesculus hippocastanum L. ˈBaumaniiˈ (syn.: A. hippocastanum L. ˈFlore Plenoˈ; A. hippocastanum ˈPlenaˈ) is a rarely cultivated cultivar characterized by fully white and sterile flowers, and as a result it does not produce fruit. For identification and characterization of the morphological, molecular, and biochemical parameters of this cultivar, we performed the pollen viability test, morphological analysis of flowers, assay of miRNA-based markers and biochemical analyses. The results show that the germination of pollen grains in artificial media reached 0 % and the pistil in flowers was undeveloped. The antioxidant activity, total polyphenol and flavonoid content exhibited the highest values of all three tested parameters (1.5540 mg TEAC/g FM; 3.7278 mg GAE/g FM and 1.328 mg CAE/g FM) in the extracts from A. hippocastanum ˈBaumaniiˈ. The applied miRNA-based markers showed a sample-specific and tissue-specific genomic polymorphism. Various DNA fingerprinting profiles between the flowers and leaf samples of fruiting and barren trees were noted. URL: https://www.upjs.sk/pracoviska/botanicka-zahrada/odborne-aktivity/contents-abstracts/

查看原文本刊更多论文

斯洛伐克稀有七叶树(Aesculus hippocastanum L. [Baumanii])的形态、分子和抗氧化分析

海马尾莲[A. hippocastanum L.]a . hippocastanum [Plena]是一种很少栽培的品种，其特点是全白色和不育的花，因此它不结果。为了鉴定和表征该品种的形态、分子和生化参数，我们进行了花粉活力试验、花形态分析、mirna标记和生化分析。结果表明，在人工培养基中花粉粒发芽率为0%，花的雌蕊发育不全。3个参数的抗氧化活性、总多酚和类黄酮含量均最高(1.5540 mg TEAC/g FM;黄芪提取物中GAE含量为3.7278 mg /g FM, CAE含量为1.328 mg /g FM。应用的基于mirna的标记显示了样本特异性和组织特异性的基因组多态性。在结果树和光秃秃树的花和叶样本之间发现了不同的DNA指纹图谱。URL: https://www.upjs.sk/pracoviska/botanicka-zahrada/odborne-aktivity/contents-abstracts/

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来源期刊

Thaiszia Journal of Botany Agricultural and Biological Sciences-Plant Science

CiteScore

1.10

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0.00%

发文量