Antagonistic activity of dominant strains of bacteria of spring barley rhizosphere against phytopathogenic micromycetes

M. Kiroiants, M. Patyka, T. Patyka
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Abstract

The purpose of the work. The aim of the study was to conduct research on the manifestation of the dominant representatives of the bacterial biome of the rhizosphere of spring barley antagonistic activity against phytopathogenic micromycetes. Research methods. The standard diffusion method of double culture in Petri dishes was used to study the antagonistic properties of the dominant strains of rhizosphere bacteria. The level of antagonistic activity of microorganisms was assessed by the indicator (%) of inhibition of growth and development of the mycelium of micromycetes Fusarium sporotrichioides Sherb. 23.2, Alternaria alternata (Fr.) Keissl. 3.45, Nigrospora oryzae (Berk. & Broome) Petch. 18.77. Results and scope of their application. Studies have shown that Bacillus methylotrophicus 10 had an inhibitory effect on Fusarium sporotrichioides Sherb. 23.2 ‒ 77,4%, Alternaria alternata (Fr.) Keissl. 3.45 – 66,6% and Nigrospora oryzae (Berk. & Broome) Petch. 18.77 – 86,7%, while the bacteria Phyllobacterium ifriqiyense 1 showed activity against phytopathogens by 45,1%, 63,1% and 65,0%, respectively. Conclusions. Thus, both strains of rhizosphere dominant bacteria have high antagonistic activity against phytopathogenic micromycetes, which gives grounds for their further study.
春大麦根际优势菌群对植物致病菌的拮抗活性研究
工作的目的。本研究的目的是研究春大麦根际细菌群落优势代表对植物病原微菌拮抗活性的表现。研究方法。采用双层培养皿标准扩散法,研究了根际优势菌株的拮抗特性。微生物拮抗活性的测定指标为:细孢毛状镰刀菌(Fusarium sporotrichiides Sherb. 23.2)、交替孢霉(Alternaria alternata, Fr.) Keissl. 3.45、米黑孢霉(Nigrospora oryzae, Berk.)菌丝生长发育抑制率(%)。(布鲁姆)佩奇,18.77。应用的结果和范围。研究表明,甲基营养芽孢杆菌10对孢子毛状镰刀菌(Fusarium sporotrichiides Sherb. 23.2 ~ 77.4%)、交替孢霉(Alternaria alternata, Fr.) Keissl. 3.45 ~ 66.6%和米黑孢霉(Nigrospora oryzae, Berk.)有抑制作用。对植物病原菌的抑菌活性分别为45.1%、63.1%和65.0%。结论。因此,两株根际优势菌对植物致病性微菌均具有较高的拮抗活性,这为其进一步研究提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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