{"title":"Electrospray and Taylor-Cone theory, Dole's beam of macromolecules at last?","authors":"Matthias S. Wilm, Matthias Mann","doi":"10.1016/0168-1176(94)04024-9","DOIUrl":null,"url":null,"abstract":"<div><p>A theoretical description of the electrostatic dispersion in electrospray is presented. The theory predicts the size of the zone at the tip of the Taylor Cone from which the liquid is ejected. To minimize this emission zone a modified electrospray ion source, the micro electrospray, has been constructed. With this ion source the size of the droplets is in the 200 nm range or below. The spray is very stable so that aqueous solutions can be dispersed in negative or positive mode without any assistance. The micro electrospray ion source is very efficient in the use of the applied amount of sample. Its flow rate of less than 25 nl/min makes MS-MS investigations on 0.5 μl of sample possible. An overall transmission coefficient of 8 x 10<sup>−4</sup> for a peptide from the solution state to the detector has been measured.</p></div>","PeriodicalId":14197,"journal":{"name":"International Journal of Mass Spectrometry and Ion Processes","volume":"136 2","pages":"Pages 167-180"},"PeriodicalIF":0.0000,"publicationDate":"1994-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0168-1176(94)04024-9","citationCount":"881","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Mass Spectrometry and Ion Processes","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0168117694040249","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 881
Abstract
A theoretical description of the electrostatic dispersion in electrospray is presented. The theory predicts the size of the zone at the tip of the Taylor Cone from which the liquid is ejected. To minimize this emission zone a modified electrospray ion source, the micro electrospray, has been constructed. With this ion source the size of the droplets is in the 200 nm range or below. The spray is very stable so that aqueous solutions can be dispersed in negative or positive mode without any assistance. The micro electrospray ion source is very efficient in the use of the applied amount of sample. Its flow rate of less than 25 nl/min makes MS-MS investigations on 0.5 μl of sample possible. An overall transmission coefficient of 8 x 10−4 for a peptide from the solution state to the detector has been measured.
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